用生物素标记的DNA探针检测尖锐湿疣和宫颈癌中的人类乳头瘤病毒DNA序列

用生物素标记的HPV6/11DNA和HPV16/18DNA探针,对尖锐湿疣和宫颈癌标本进行原位杂交。结果大多数尖锐湿疣含HPV6/11DNA,所以尖锐湿疣的主要致病因子是H2V6/11.HPV6/11DNA阳性细胞主要位于上皮的表层,少数位于深层。个别合并HPV16/18感染,或仅为HPV16/18所感染的病人恶变的可能性大。大多数宫颈癌病人含HPV16/18DNA,提示宫颈癌的主要致病因子为HPV16/18。一些病人合并HPV6/11感染。在未找见HPV16/18DNA的病例中,可能存在其它致癌因子。

Use of Biotinylated DNA Probes in Detecting Condyloma Acuminata and Cervical Carcinoma for Human Papillomavirus DNA Sequences

In situ hybridization is a valuable method for the demonstration of the presence, localization and distribution of viral DNAS within frozen histological sections or paraffin embedded sections. Using biotin-lablled HPV6/11 DNA and HPV16/18 DNA as prodes, we studied specimens from condyloma acuminata and cervical carcinoma with in situ hybridization method. It has been shown that most of the condyloma acuminata specimens contained HPV6/11 DNA So the etiologic factor of condyloma acuminata may be HPV6/11. The HPV 6/11 DNA positive nuclei mainly located in the upper part of the epithelium, with some scattering in the deeper part. This suggests that HPV6/11 may first infect deeper part of the epithelium and the replication of viral DNA is completed in the upper part. A few condyloma acuminata patients appeared to have mixed infection, containing both HPV6/11 DNA and HPV16/18 DNA. These patients were probably at the risk of progression to carcinoma. With regard to cervical carcinoma, a high proportion of these patients contained HPV16/18 DNA. This suggests that the major etiologic factor of cervical carcinoma may be HPV 16/18. Some patients infected with both HPV 16/18 and HPV6/11. In a few cases, HPV 16/18 DNA were not found, there should be other factors responsible for the malignancy of this kind of patients.