| 分光光度法测定鱿鱼生殖腺提取物中总磷脂 |
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| 引用本文： | 袁延强,侯海荣,王希敏,何秋霞,韩利文,刘可春.分光光度法测定鱿鱼生殖腺提取物中总磷脂[J].现代药物与临床,2011,26(1):63-65. |
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| 作者姓名： | 袁延强 侯海荣 王希敏 何秋霞 韩利文 刘可春 |
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| 作者单位： | 山东省科学院生物研究所 |
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| 基金项目： | 国际合作项目(2007DFR30140) |
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| 摘 要： | 目的建立鱿鱼生殖腺提取物中总磷脂的测定方法。方法采用乙醇提取、正己烷萃取法得到鱿鱼生殖腺提取物,浓硫酸-高氯酸法消化样品,抗坏血酸-钼蓝法测定无机磷的量,再乘以系数26.31折算成鱿鱼生殖腺提取物中总磷脂的量。结果磷在0.2～1.0μg/mL线性关系良好(r=0.9999),平均回收率为95.06%,RSD为1.09%。结论该方法方便、快速、准确度较高,可用于鱿鱼生殖腺提取物中总磷脂的测定。
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| 关 键 词： | 鱿鱼生殖腺 总磷脂 浓硫酸-高氯酸消化法 抗坏血酸-钼蓝法 分光光度法 |
| 收稿时间： | 2010/10/15 0:00:00 |
Determination of total phospholipids in Ommastrephes bartrami gonad extracts by spectrophotometry |
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| YUAN Yan-qiang and HOU Hai-rong,WANG Xi-min,HE Qiu-xi,HAN Li-wen,LIU Ke-chun.Determination of total phospholipids in Ommastrephes bartrami gonad extracts by spectrophotometry[J].Drugs & Clinic,2011,26(1):63-65. |
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| Authors: | YUAN Yan-qiang and HOU Hai-rong WANG Xi-min HE Qiu-xi HAN Li-wen LIU Ke-chun |
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| Institution: | Biology Institute of Shandong Academy of Sciences,Jinan 250014,China |
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| Abstract: | Cinobufacini Injection is the second class new drug independently developed by China, which is water-soluble preparations processed by the main component of Bufo bufo gargarizans skin. As a kind of Chinese materia medica with antitumor effect, Cinobufagin Injection is widely used in clinical practice, especially in anti-liver cancer. It could not only inhibit and kill various kinds of hepatoma carcinoma cells, but also be satisfactory effective in treating patients with liver cancer either when applied lonely or together with interventional therapy. This paper will rewiew the latest progress on the basic and clinical application in treating liver cancer of Cinobufagin Injection.. |
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| Keywords: | Cinobufagin Injection hepatoma carcinoma cell antitumor clinical application |
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