芪药消渴胶嚢对高脂饮食诱导追赶生长大鼠肝脏及骨骼肌糖脂代谢的影响

目的观察益气滋阴中药芪药消渴胶囊对高脂饮食诱导追赶生长大鼠肝脏及骨骼肌糖脂代谢的影响。方法采用限食及开放高脂膳食法复制追赶生长大鼠模型,用不同剂量的芪药消渴胶囊1.8g/(kg.d),0.6g/(kg.d)]干预,并与吡格列酮组比较,8周后观察各组大鼠血糖、血清游离脂肪酸(FFA)、血清胰岛素(Fins)、胰岛素抵抗指数(insulin resistance index,IRI)、血浆脂联素、附睾及肾周脂肪重量及与体重相对比值,肝脏及骨骼肌脂质总胆固醇(TC)、甘油三酯(TG)含量及葡萄糖摄取率。结果高脂饮食可诱导追赶生长大鼠胰岛素抵抗,与模型组比较,芪药消渴胶囊可降低高脂饮食诱导追赶生长大鼠血清Fins及IRI(P0.01,P0.05);芪药消渴胶囊还能明显降低高脂饮食诱导追赶生长大鼠血中FFA水平,升高血浆脂联素含量,与模型组比较差异有统计学意义(Р0.01,P0.05);大剂量芪药消渴胶囊组还能降低肝脏及骨骼肌TG含量,促进骨骼肌葡萄糖摄取率,其疗效与吡格列酮组相似(P0.05)。结论芪药消渴胶囊可通过不同的干预途径改善高脂饮食诱导追赶生长大鼠的葡萄糖代谢水平和(或)脂代谢以减轻或延缓肝脏尤其是骨骼肌胰岛素抵抗的发生和发展。

Effects of Qiyao Xiaoke Capsule on Glycolipid Metabolism in Liver and Skeletal Muscle of Rats with Catch-up Growth Induced by High-fat Diet

Objective To observe the effect of Qiyao Xiaoke Capsule（QXC,a Chinese preparation for invigorating qi and nourishing yin）on glycolipid metabolism in the liver and skeletal muscle of rats with catch-up growth（CUG）induced by high-fat diet.Methods CUG model rats were made by limiting forge follouwed by high-fat diet,and intervened with different dosages 〔1.8（g/kg·d）,0.6 g/（kg·d）〕 of QXC.And the experiment was controlled with pioglitazone.Levels of blood glucose,serum free fatty acid（FFA）,serum insulin（Fins）,plasma adiponcetin（AC）and insulin resistance index（IRI）as well as the weights of peri-epididymis and peri-renal fat and their ratio to body weight were observed 8 weeks later.The contents of total cholesterol（TC）,triglyceride（TG）and glucose uptake in skeletal muscle and liver were also determined.Results Insulin resistance in CUG rats can be induced by high-fat diet.Compared with the un-treated model rats,levels of Fins and AC were higher,IRI and FFA were lower in CUG rats after intervened by QXC（P0.05 or P0.01）.Moreover,QXC at large dose showed the effects of reducing TG content in liver and skeletal muscle,advancing the glucose uptake in skeletal muscle,displaying an efficacy similar to that of pioglitazone（P0.05）.Conclusion QXC could improve the glycolipid metabolism in high-fat diet-induced CUG rats through different pathways of intervention to alleviate/delay the occurrence and development of insulin resistance in the liver and skeletal muscle.