雌激素诱导C57BL/6妊娠小鼠肝损伤的比较研究

目的比较雌激素对C57BL/6同种系和异种系妊娠小鼠肝脏的影响,探讨妊娠期肝损伤小鼠模型选择C57BL/6异种系妊娠小鼠的可能性。方法选择健康C57BL/6雄鼠、BALB/c雄鼠分别和C57BL/6雌鼠交配,得到C57BL/6同/异种系妊娠小鼠,并分为对照组、溶剂组和不同剂量给药组。给药组从怀孕10 d起连续4 d分别每天皮下注射不同剂量17-α-乙炔雌二醇。结果给药的异种系孕鼠血清中除碱性磷酸酶(ALP)指标外丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)和甘胆酸(CG)升高水平与同种系孕鼠比较差异有统计学意义(P<0.05),其肝脏组织病理学结果也不同,且剂量为1.0μg/g体重的异种系孕鼠肝脏NK细胞出现集聚和活化现象。结论雌激素对C57BL/6同种系和异种系妊娠小鼠肝脏作用不同,选择雌激素诱导C57BL/6异种系妊娠小鼠建立妊娠期肝损伤模型能够反映该病的肝脏免疫学变化。

Comparative study on liver injury of syn-/allogeneically pregnant mice

Objective To observe the Influence of estrogen on the livers of syn-/allogeneically pregnant(C57BL/6) mice in order to establish a valid mouse model of liver injury of pregnancy.Methods C57BL/6 and BALB/c male mice were randomly mated with C57BL/6 female mice to obtain syn-/allogeneically pregnant C57BL/6 mice.The pregnant mice were divided into control,solvent and drug-given groups.The drug-given group were injected subcutaneously 17-α-ethinylestradiol from 10d of gestation for 4 days.Results Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and glycocholic acid(CG)were significantly elevated in the allogeneically pregnant mice,compared as syngeneically pregnant mice(P<0.05),but alkaline phosphatase(ALP)didn't show so much difference(P>0.05).The results of hepatic histopathology also showed difference,and NK cells accumulated and markedly activated only in the liver of 1.0 μg/g body wt drug-given allogeneically pregnant mice.Conclusion The effects of estrogen on the livers of syn-/allogeneically pregnant C57BL/6 mice were different,and estrogen-induced allogeneically pregnant C57BL/6 mice as the animal model of liver injury of pregnancy can reveal the liver immunological change of the disease.