灯盏细辛对急性期大鼠脑缺血再灌注后细胞凋亡影响作用的研究

目的研究灯盏细辛对急性期大鼠脑缺血再灌注后细胞凋亡的影响作用。方法将健康SD大鼠65只分成3组,分别为正常组（5只）、脑缺血再灌注模型组（20只）、灯盏细辛治疗组（20只）。除正常组外,模型组和治疗组设2个时间点,即3 d与7 d,每个时间点10只。利用采用改良的Zealonga法建立大鼠大脑中动脉局灶缺血,即再灌注梗死（MCAO）模型,分别于脑缺血再灌注3 d和7 d进行神经病学评分和脑组织含水量测定,用苏木精-伊红（HE）进行形态学观察,用TUNEL法检测脑组织凋亡细胞的变化。结果再灌注3 d和7 d神经病学评分和脑组织含水量测定,治疗组Bederson评分均高于模型组（P〈0.05）;治疗组大鼠脑组织含水量与模型组比较均减小（P〈0.05）。结论灯盏细辛对急性期大鼠脑出血再灌注后细胞凋亡有明显的抑制作用,对促进受损脑组织的恢复作用明显。

Effect of the expression of apoptosis in beviscapine applied to rats with cerebral ischemia reperfusion injury

Objective To investigate the effect of breviscapin on rats with cerebral ischemia reperfusion injury. Methods A total of 65 healthy SD rats were divided randomly into three groups ： normal group （ n = 5 ）, cerebral ischemia reperfusion group （ n = 20） and beviscapine group （n =20）. The rats in normal group were subdivided into 3 - day and 7 - day with （n = 10）. The middle cerebral artery occlusion/reperfusion （MCAO） model was established using of improved Zealonga. The neurological score and brain water contents of the MCAO were evaluated at 3d and 7d：and the histological changes were observed through Hematoxylin - Eosin staining, and the cell apoptosis were detected by TUNEL method. Results Beviscapine reduced the score of severity of MCAO rats＇nervous system disease,the degree of brain edema and the number of apoptotic cells （P 〈 0.05）. Conclusion Breviscapine can inhibit apoptotic cells in cerebral ischemia reperfusion injury, and get an obvious effect in promoting the recovery of injured brain.