开心胶囊抗大鼠心室重构的分子机制研究

目的:观察开心胶囊对心肌梗死大鼠心脏形态、舒张收缩功能变化的影响,探讨蛋白激酶C在心室重构过程中的作用。方法:采用结扎大鼠左冠状动脉方法制作大鼠心肌梗死模型,随机分为开心胶囊组、卡托普利组、模型组、假手术组;开心胶囊组10 g/kg.d-1开心胶囊溶液灌胃,卡托普利组1 g/L卡托普利混悬液10 ml/kg.d-1灌胃,假手术组和模型组正常饮水。常规饲养14周后,多普勒超声心动图评价大鼠心功能,大鼠心肌结缔组织染色,检测心肌细胞胞浆蛋白激酶C活性。结果:模型组大鼠心功能明显低于其他3组,心肌肥大、纤维化程度、非梗死区心肌细胞胞浆PKC活性明显高于其他3组(P<0.01)。开心胶囊组与卡托普利组对比心功能、心肌纤维化染色结果及心肌细胞内的PKC活性均无显著性意义(P>0.05)。结论:开心胶囊抗心肌梗死后心室重构作用可能与其调节心肌细胞内的PKC活性有关,作用与卡托普利无明显差异。

The molecular mechanism of Kaixin capsule on ventricular remodeling in rat with myocardial infarction

Objective:To study the effects of Kaixin capsule on ventricular remodeling in rat with myocardial infarction(MI).And the function of protein kinase C(PKC) has also been explored.Methods: Wistar rats which were ligated of the left main coronary artery to establish model of MI were randomly divided into 3 groups:Kaixin capsule group,captopril group and model group,12 rats in each group.Additional 12 healthy female Wistar rats were selected as the sham-operation group which was treated the same as the other groups except the arteria coranaria ligated operation.Rats in Kaixin capsule group were given gastric perfusion of 10 g/kg·d-1 of Kaixin capsule.Rats in captopril group received gastric perfusion of 10 ml/kg·d-1 of 1 g/L suspension of captopril.Rats in sham-operation group and model group were allowed to drinking freely.After 14 weeks of continuous administration,the heart function of rats in each group were evaluated by Doppler echocardiography.Mallory trichrome staining method was used to stain the connectivetissue of cardiac muscle.The PepTag(r) Assay for non-radioactive detection of PKC was used to assess PKC activity of cardiac muscle cells cytoplasm.Results: The heart function of model group was significantly lower than the other 3 groups.The cardiac muscle cells of model group were hypertrophy and the degree of cardiac muscle interstitial fibrosis was serious.The PKC activity of model group rats were significantly higher than the other 3 groups(P<0.01).There was no significant difference between compound Chinese medicine group and captopril group in heart function,result of Mallory's trichrome staining and PKC activity of the cardiac muscle cells cytoplasm(P>0.05).Conclusion:Myocardial infarction can lead to ventricular remodeling.Kaixin capsule has the same effects as captopril on anti-remodeling of ventricle after myocardial infarction.The related mechanism is adjusting the PKC activity of the cardiac muscle cells cytoplasm.