肾病综合征大鼠中TNF-α、HA与CD44相关性的实验研究

研究细胞因子 (肿瘤坏死因子TNF α )、细胞间质成分 (透明质酸HA )与细胞粘附分子 (CD4 4 )在肾病综合征中的关系及作用。经尾静脉一次性注射阿霉素构建肾病综合征大鼠模型 ,注射阿霉素 14d ,大鼠肾病综合征模型建立。采用放射免疫法及流式细胞术检测TNF α、HA及外周血单核细胞CD4 4阳性细胞数。结果显示模型组血清及肾组织TNF α、HA浓度明显高于对照组 (P <0 0 1)。CD4 4阳性率显著增高 ,与对照组比较有显著性差异 (P <0 0 1) ,肾病综合征CD4 4表达上调与HA浓度增高呈正相关 ,HA与TNF α亦呈正相关。CD4 4与TNF α、HA共同参与了肾病综合征的发病过程。

Correlations between TNF-α,Hyaluronic Acid and CD44 in Experimental Model of Rats with Nephrotic Syndrome

To investigate the correlations between TNF α,hyaluronic acid(HA) and CD44 a experimental model of rats with nephrotic syndrome(NS) was induced by a sigle injection of Adriamycin(ADM) through tail vein,and the contents of TNF α and HA as well as the numbers of CD44 positive cells in peripheral blood mononuclear cells were determined by radio immunoassay and flow cytometry analysis The experimental results showed that the concentrations of TNF α and HA in sera and renal tissues of the experimental groups were significantly higher than those of normal controls,and the numbers of CD44 positive cells also increased obviously These results suggest that the up regulation of CD44 positively correlates with increase of HA concentration in ANS,and CD44,HA and TNF α participate the development of nephrotic syndrome