| 舒心饮预给药对急性心肌缺血大鼠心肌Fas的影响 |
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| 引用本文： | 励冬斐,孙鑫,胡丹丹,孙勇,章怡祎,梁知,陈伟. 舒心饮预给药对急性心肌缺血大鼠心肌Fas的影响[J]. 上海中医药大学学报, 2011, 0(6): 63-66 |
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| 作者姓名： | 励冬斐 孙鑫 胡丹丹 孙勇 章怡祎 梁知 陈伟 |
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| 作者单位： | 上海中医药大学附属龙华医院;上海市浦东医院; |
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| 基金项目： | 上海市科委科研计划基金资助项目(08411961400) |
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| 摘 要： | 目的:探讨舒心饮预给药对急性心肌缺血大鼠心肌细胞凋亡抑制因子(Fas)的影响,为舒心饮的临床应用提供依据。方法:将40只Wistar健康雄性大鼠随机分为对照组、模型组、舒心饮组、单硝酸异山梨酯组。对照组、模型组给予灌胃蒸馏水,舒心饮组灌胃2.43 g/kg生药,单硝酸异山梨酯组灌胃1 mg/kg。用药5周后,结扎左冠状动脉前降支造模,观察大鼠血清肌酸磷酸激酸(CPK)及乳酸脱氢酶(LDH)浓度,RT-PCR检测心肌肿瘤坏死因子-α(TNF-α)、Fas mRNA,Western blot检测心肌Fas、半胱氨酸蛋白酶-3(Caspase-3)表达水平,评价舒心饮对大鼠急性心肌缺血的作用。结果:舒心饮可明显降低血CPK、LDH浓度,下调TNF-α、Fas转录水平及Fas、Caspase3蛋白水平的表达。结论:舒心饮预处理对大鼠急性缺血心肌有保护作用,下调死亡途径的受体和配体从而拮抗心肌组织的凋亡可能是其作用机制之一。
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| 关 键 词： | 舒心饮 急性心肌缺血 细胞凋亡抑制因子 半胱氨酸蛋白酶 |
Effects of Shuxin Decoction Preconditioning on Fas in Myocardium in Rats of Acute Myocardial Ischemia |
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| LI Dong-fei, SUN Xin HU Dan-dan SUN Yong ZHANG Yi-yi LIANG Zhi CHEN Wei. Effects of Shuxin Decoction Preconditioning on Fas in Myocardium in Rats of Acute Myocardial Ischemia[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2011, 0(6): 63-66 |
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| Authors: | LI Dong-fei SUN Xin HU Dan-dan SUN Yong ZHANG Yi-yi LIANG Zhi CHEN Wei |
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| Affiliation: | LI Dong-fei1,2 SUN Xin1 HU Dan-dan1 SUN Yong1 ZHANG Yi-yi1 LIANG Zhi1 CHEN Wei1 1.Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine 2.Shanghai Pudong Hospital |
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| Abstract: | Objective: To observe the effects of Shuxin Decoction on Fas in myocardium in rats of acute myocardial ischemia,in order to provide reference for clinical application of Shuxin Decoction.Methods: Forty male Wistar rats were randomly divided into 4 groups with 10 rats in each group: control group,model group,Shuxin Decoction group and isosorbide mononitrate group.The rats in Shuxin Decoction group and isosorbide mononitrate group were administered intragastrically with Shuxin Decoction 2.43 g/kg and isosorbi... |
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| Keywords: | Shuxin Decoction acute myocardial ischemia Fas Caspase |
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