| 黄芪多糖对兔脂肪来源的间充质干细胞体外增殖的影响 |
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| 引用本文： | 何文涓,张兰芳,袁志坚,何晓升.黄芪多糖对兔脂肪来源的间充质干细胞体外增殖的影响[J].中国生化药物杂志,2011,32(5). |
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| 作者姓名： | 何文涓 张兰芳 袁志坚 何晓升 |
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| 作者单位： | 1. 无锡卫生高等职业技术学校,江苏,无锡,214028
2. 杭州师范大学,临床医学院,浙江,杭州,310036 |
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| 基金项目： | 江苏省无锡市科技局科技支撑计划立项(CSZ01047) |
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| 摘 要： | 目的黄芪多糖(APS)对兔脂肪来源的间充质干细胞(ASCs)体外增殖的作用。方法提取兔ASCs进行培养,设实验组和对照组,实验组加入不同浓度APS注射液,对照组加常规培养液,四甲基偶氮唑盐(MTT)法观察细胞的增殖率,比较两组之间的差异。结果 APS浓度为1.95～3.90μg/mL时,对ASCs的增殖无明显影响(P>0.05);而浓度为7.80～1 000μg/mL时,对ASCs的增殖有明显抑制作用(P<0.01);在一定浓度范围内(25～100μg/mL),浓度对细胞增殖的影响不大,而与培养的时间有关,随着作用时间的延长,对ASCs的抑制作用更加明显。结论低浓度APS对兔ASCs体外增殖无影响;高浓度APS对ASCs有明显的抑制作用。
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| 关 键 词： | 黄芪多糖 脂肪来源的间充质干细胞 体外增殖 |
Effects of astragalus polysaccharide on in vitro proliferation of rabbit adipose-derived mesenchymal stem cells |
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| HE Wen-juan,ZHANG Lan-fan,YUAN Zhi-jian,HE Xiao-sheng.Effects of astragalus polysaccharide on in vitro proliferation of rabbit adipose-derived mesenchymal stem cells[J].Chinese Journal of Biochemical Pharmaceutics,2011,32(5). |
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| Authors: | HE Wen-juan ZHANG Lan-fan YUAN Zhi-jian HE Xiao-sheng |
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| Institution: | HE Wen-juan1,ZHANG Lan-fan1,YUAN Zhi-jian1,HE Xiao-sheng2(1.Wuxi Higher Health Vocational Technical School,Wuxi 214028,China,2.Clinical School of Medicine,Hangzhou Normal University,Hangzhou 310036,Chian) |
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| Abstract: | Purpose To investigate the effects of astragalus polysaccharide(APS) on proliferation of rabbit adipose-derived mesenchymal stem cells(ASCs) in vitro.Methods ASCs were obtained from an experimental rabbit and cultured.Multi experimental group and a control group were designed.Different concentrations of APS were added to the culture fluid in the experimental group,while a conventional culture method was employed in the control group.The proliferation rates of ASCs were evaluated using MTT method,and compare... |
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| Keywords: | astragalus polysaccharide rabbit adipose-derived mesenchymal stem cells in vitro proliferation |
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