| microRNA-148a对前列腺癌细胞系LNCaP神经内分泌分化的影响 |
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| 引用本文： | 邓震,矫力,蔡小兵,张振声,曾钦松,许传亮,孙颖浩. microRNA-148a对前列腺癌细胞系LNCaP神经内分泌分化的影响[J]. 上海医学, 2011, 34(7) |
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| 作者姓名： | 邓震 矫力 蔡小兵 张振声 曾钦松 许传亮 孙颖浩 |
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| 作者单位： | 1. 第二军医大学附属长海医院泌尿外科, 上海,200433
2. 总参谋部警卫局保健处 |
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| 摘 要： | 目的探讨microRNA(miRNA)-148a对前列腺癌细胞系LNCaP神经内分泌分化的影响。方法采用实时定量聚合酶链反应(RT-PCR)验证miRNA-148a在雄激素依赖性与雄激素非依赖性前列腺癌细胞系中的差异表达。采用miRNA-148a及其抑制物(anti-miRNA-148a)改变LNCaP细胞中miRNA-148a的表达量,观察LNCaP细胞神经内分泌分化的差异程度,并检测神经内分泌分化标志物神经元特异性烯醇化酶(NSE)的差异表达。结果 LNCaP-AI细胞中的miRNA-148a相对表达量显著低于LNCaP细胞(P<0.05)。下调miRNA-148a在LNCaP细胞中的表达量,可明显促进LNCaP细胞神经内分泌分化。结论 miRNA-148a可以抑制LNCaP细胞的神经内分泌分化,可能成为前列腺癌生物治疗的新靶点。
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| 关 键 词： | 前列腺癌 microRNA-148a 神经内分泌分化 |
Effect of microRNA-148a on neuroendocrine differentiation of human prostate cancer cell line LNCaP |
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| DENG Zhen,JIAO Li,CAI Xiaobing,ZHANG Zhensheng,ZENG Qinsong,XU Chuanliang,SUN Yinghao. Effect of microRNA-148a on neuroendocrine differentiation of human prostate cancer cell line LNCaP[J]. Shanghai Medical Journal, 2011, 34(7) |
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| Authors: | DENG Zhen JIAO Li CAI Xiaobing ZHANG Zhensheng ZENG Qinsong XU Chuanliang SUN Yinghao |
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| Affiliation: | DENG Zhen,JIAO Li,CAI Xiaobing,ZHANG Zhensheng,ZENG Qinsong,XU Chuanliang,SUN Yinghao.Department of Urology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | Objective To detect the effect of microRNA-148a on the neuroendocrine differentiation of human prostate cancer cell line LNCaP.Methods The differential expression of microRNA(miRNA-148a) in androgen-dependent prostate cancer(ADPC) and androgen-independent prostate cancer(AIPC) cell lines was examined by real time(RT)-PCR.miRNA-148a and anti-miRNA-148a were used to affect the expression of miRNA-148a in LNCaP cells.Neuroendocrine differentiation of LNCaP cells was observed under inverted microscope.The level... |
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| Keywords: | Prostate cancer MicroRNA-148a Neuroendocrin differentiation |
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