| 青蒿叶片愈伤组织的诱导和植株再生 |
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| 引用本文： | 伍晓丽,刘飞,李隆云,钟国跃. 青蒿叶片愈伤组织的诱导和植株再生[J]. 时珍国医国药, 2007, 18(5): 1106-1107 |
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| 作者姓名： | 伍晓丽 刘飞 李隆云 钟国跃 |
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| 作者单位： | 重庆市中药研究院,重庆,400065 |
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| 基金项目： | 重庆市科委科技攻关项目 |
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| 摘 要： | 目的探讨以青蒿幼嫩叶片再生植株的方法。方法以青蒿幼嫩叶片为外植体,接种于附加不同激素组合的MS培养基,进行愈伤组织诱导和植株再生。结果以6-BA0.1 mg/L 2,4-D0.3 mg/L诱导愈伤组织,在含6-BA0.1 mg/L IAA0.3 mg/L的培养基上分化,用含IAA0.1 mg/L NAA0.3 mg/L的培养基生根。结论用以上方法可以在较短周期内从幼嫩叶片得到再生青蒿植株。
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| 关 键 词： | 青蒿 植物激素 愈伤 再生 |
| 文章编号： | 1008-0805(2007)05-1106-02 |
| 修稿时间： | 2006-09-08 |
The Induction of Callus from Leaf of Artemisia annua L. and its Regeneration |
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| WU Xiao-li,LIU Fei,LI Long-yun,ZHONG Guo-yue. The Induction of Callus from Leaf of Artemisia annua L. and its Regeneration[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(5): 1106-1107 |
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| Authors: | WU Xiao-li LIU Fei LI Long-yun ZHONG Guo-yue |
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| Abstract: | Objective To explore the way of regeneration of plants from leaves of Artemisia annua L..Methods To plant the leaves of Artemisia annua L.on mediums plus different plant hormones.Results The suitable callus-inducing medium was MS 6-BA1.0 mg/L 2,4-D0.3 mg/L.The suitable regenerating medium was MS 6-BA0.1 mg/L IAA0.3 mg/L.The suitable root-inducing medium was MS IAA0.1 mg/L NAA0.3 mg/L.Conclusion Young plants can regenerate from leaves in a short span by the method above. |
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| Keywords: | Artemisia annua L Plant hormones Callus Regeneration |
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