| 幽门螺杆菌粘附素基因hpaA研究 |
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| 引用本文： | 洪愉,毛旭虎,刘正祥,井申荣,邹全明.幽门螺杆菌粘附素基因hpaA研究[J].中华消化外科杂志,2003,2(5):331-335. |
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| 作者姓名： | 洪愉 毛旭虎 刘正祥 井申荣 邹全明 |
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| 作者单位： | 1. 成都军区昆明总医院核医学科
2. 400038,第三军医大学生物医药中试研究基地暨医学检验系统临床微生物学与免疫学教研室 |
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| 基金项目： | 国家重点科技攻关计划项目(96-61-01-54)，“863”生物与现代农业技术领域生物工程技术主题课题(2001AA215161) |
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| 摘 要： | 目的 克隆表达幽门螺杆菌鞭毛粘附素基因hpaA,筛选Hp疫苗有效抗原成分。方法采用PCR技术从幽门螺杆菌基因组中扩增hpaA基因,克隆至表达载体pET-28a(+)中,IPTG诱导表达重组蛋白HpaA(rHpaA),鉴定rHpaA蛋白的表达形式。结果 pPHA重组菌以包涵体形式表达rH-paA,表达量约占全菌的35％。纯化后的rHpaA免疫家兔获得了高效价的兔抗HpaA抗血清,且该抗血清能够与不同Hp菌株中相应分子量条带发生抗原抗体反应,并能在一定程度上抑制由Hp引起的RBC凝集现象。结论 Hp鞭毛粘附素HpaA在重组大肠杆菌中能够高效表达,且具有良好的免疫反应性。
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| 关 键 词： | 幽门螺杆菌 hpaA E.coli 克隆 表达 |
| 文章编号： | 1671-4555(2003)05-0331-05 |
| 修稿时间： | 2003年7月2日 |
Research on an Adhesin Gene hpaA of Helicobacter pylori |
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| Hong Yu,Mao Xuhu,Liu Zhengxiang,Jing
Shenrong,Zou Quanming. Pilot Scale Research Center for Biopharmaceutics &.Research on an Adhesin Gene hpaA of Helicobacter pylori[J].Chinese Journal of Digestive Surgery,2003,2(5):331-335. |
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| Authors: | Hong Yu Mao Xuhu Liu Zhengxiang Jing
Shenrong Zou Quanming Pilot Scale Research Center for Biopharmaceutics & |
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| Institution: | Hong Yu,Mao Xuhu,Liu Zhengxiang,Jing
Shenrong,Zou Quanming. Pilot Scale Research Center for Biopharmaceutics & Department of Clinical Microbiology and Immunology,College of Medical Laboratory Sciences,Third Military Medical University,Chongqing 400038 |
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| Abstract: | Objective To clone and express adhesin gene hpaA of Helicobacter pylori and select the effective candidate for Hp vaccine. Methods Gene hpaA amplified from Hp genome was cloned to pET- 28a( + ) vector, then the recombinant strain (pPHA/BL21) was induced to express rHpaA by IPTG. Results rHpaA occupied about 35 % in total thallus protein of pPHA recombinant strain. By way of immunizing rabbit with purified rHpaA, a high - liter of rabbit anti - HpaA serum has been gained which could react to the corresponding molecular weight band of different Hp strains and could block the hemagglutination caused by Hp . Conclusions HpaA of Hp could be generously expressed in the recombinant E. coli and retained satisfied immunoreaclive. |
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| Keywords: | Helicobacter pylori hpaA E coli clone expression |
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