小鼠颅神经嵴细胞的培养和特征

目的：在体外原代培养Balb/c小鼠胚胎的颅神经嵴细胞。为颅面部各种组织细胞的发育研究提供细胞来源。方法：采用胰酶消化法分离小鼠胚胎第8.5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞，用免疫组织化学方法鉴定细胞的来源，并测定细胞的生长曲线。结果：成功地培养出小鼠的颅神经嵴细胞，其形态类似成纤维样细胞，免疫组化检测结果表明，神经特异性烯醇化酶（NSE）抗体染色结果阳性。细胞的群体倍增时间为43.65h。结论：原代培养的小鼠颅神经嵴细胞生长稳定，来源明确，是颅面部各种细胞的发育和分化研究中一种有用的工具。

Culture and characterization of murine cranial neural crest cells

AIM: The purpose of the present study is to culture cranial neural crest cells in vitro . Many types of cells were developed from this cell line in the orofacial organ and tissue development. METHODS: Cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells. The characterization of the cells were detected by immunohistochemical analysis and growth curves. RESULTS: Cranial neural crest cells were cultured successfully. The shape of the cells were fibroblast-like. The results of immunohistochemical examination showed that neural crest cells stained neuron-specific enolase (+). The population double time of the cells was 43.65h. CONCLUSION: Growth of the murine cranial crest cells were stable during culture and their sources were correct, providing a useful tool for molecular studies of development and cell differentiation in cranial region and oral cavity.