正常和双眼形觉剥夺大鼠发育过程中视皮层神经元NR1的表达

目的 在视觉发育可塑性关键期内,探讨双眼形觉剥夺对大鼠视皮层神经元N-甲基-D-天冬氨酸受体亚单位1(N-methyl-D-aspartate receptor 1,NR1)的影响。方法对出生后14、21和28 d龄正常、双眼形觉剥夺和去除剥夺大鼠视皮层脑片进行免疫细胞化学染色。结果 正常组大鼠睁眼后,视皮层NR1阳性神经元的相对密度随天龄增加而逐渐减小(P<0.05),双眼形觉剥夺和去除剥夺组大鼠视皮层的这一相对密度亦随天龄增加而逐渐减小(P<0.05)。结论在视觉发育可塑性关键期内,大鼠视皮层神经元NR1的表达呈年龄依赖性变化,不受双眼形觉剥夺影响。

NR1 expression of visual cortical neurons in normal and binocular form-deprived rats during development

Objective To investigate the effects of binocular form-deprivation on the expression of N-methyl-D-aspartate receptor subunit 1 ( NR1) of visual cortical neurons in rats during the critical period of visual plasticity. Methods Immunocytochemical staining of the visual cortex slices of normal rats, binocular form-deprived rats and removed binocular form-deprivation rats at postnatal days 14, 21 and 28 was performed. Results After eye-opening, the relative density of NRl positive neurons in the visual cortex of normal rats, binocular form-deprived rats and removed binocular form-deprivation rats decreased in an age-dependent manner( P < 0.05). Conclusion During the critical period of visual development, the expression of NR1 in rat visual cortical neurons changes in an age-dependent manner. Binocular form-deprivation has no effect on the expression of NR1.