饮食对胰岛素抵抗大鼠肝脏组织中PTP 1B表达的影响

目的在高脂饮食诱导胰岛素抵抗的基础上,观察饮食对大鼠肝脏中蛋白酪氨酸磷酸酶1B(PTP1B)表达的影响.方法选取雄性Wistar大鼠30只,随机分为正常对照组10只,给予基础饲料;模型组20只,给予高脂饲料.模型组大鼠给予高脂喂养4周后,随机分为2亚组胰岛抵抗组,继续高脂饮食;饮食组,给予低脂饮食干预.干预6周后,用蛋白印迹法检测各组大鼠肝组织中PTP1B蛋白含量.结果在胰岛素抵抗状态时,大鼠肝组织中PTP1B蛋白与对照组相比,含量增加98.3%(t=9.335,P＜0.001);饮食干预能明显降低PTP1B的表达,与胰岛素抵抗组相比减少32.7%(t=4.076,P＜0.001).结论饮食干预能改善机体胰岛素抵抗,可能与降低肝脏组织中PTP1B蛋白表达有关.

The Effect of Diet on Expression of Protein Tyrosine Phosphatase 1B in Liver of Insulin Resistant Rats

Objective To investigate the effect of diet on the expression of protein tyrosine phosphatase (PTP) 1B in liver of insulin resistant rats induced by high fat diet. Methods 30 male Wistar rats were randomly divided into the control group (n=10) which was given basic diet, and the model group (n=20) which was given high fat diet. After 4 weeks, the model group was randomly divided into 2 subgroups:insulin resistant group which continued to receive high fat diet, and diet-treated group which accepted diet intervention for 6 weeks. The protein expression level of PTP1B in the liver of rats were determined with western blotting at the end of 10th week. Results The protein content of PTP1B in the liver of insulin resistant rats significantly increased by 98.3% compared with the control group (t=9.335,P<0.001). After 6-week diet treatment, the protein level of PTP1B in the liver of diet-treated group obviously decreased by 32.7% compared with insulin resistant group(t=4.076,P<0.001). Conclusion Diet treatment could improve insulin resistance possibly by reducing the protein expression of PTP 1B in the liver.