恶性成釉细胞瘤定量病理学研究

目的:探讨恶性成釉细胞瘤细胞核形态参数、DNA含量及AgNOR形态分型和计数在诊断中的意义。方法: 选取17例恶性成釉细胞瘤患者的切除标本行HE、AgNOR及DNA染色,应用图像分析仪对细胞核形态几何参数、 DNA含量及倍体、AgNOR进行定量分析。结果:在细胞核的几何参数中,恶性成釉细胞瘤细胞的面积、周长、等圆直径、短径、平均直径、圆度、轴比与良性成釉细胞瘤均有显著性差异(P<0101)。恶性成釉细胞瘤AgNOR计数和 DNA指数显著高于良性成釉细胞瘤(P<0101)。根据核形态参数和DNA指数建立了判别恶性成釉细胞瘤的回归方程。结论:细胞核DNA含量和形态参数的测定以及AgNOR分型和计数有助于恶性成釉细胞瘤的鉴别诊断。

A Quantitatively Pathological Study on Malignant Ameloblastomas

Objective: The purpose of this study was to investigate the role of nuclear morphometric parameters, DNA content and AgNOR count in the differentiating malignant and benign ameloblastomas.Methods: Totally 17 cases of malignant ameloblastomas were examined by using HE, AgNOR and DNA stain methods. Morphometric parameters of cell nuclei, DNA content and AgNOR count were quantitatively studied by using an image analysis system.Results: Seven parameters (area, perimeter, equal diameter, minor diameter, mean diameter, round index, axis ratio) out of ten shape factors were significantly different between malignant and benign ameloblastoma (P<0 01). AgNOR count and DNA index in malignant ameloblastoma were significantly higher than those in benign ameloblastoma (P<0 01). Logistic regression equation was established, according to nuclear morphormetric parameters and DNA index.Conclusion: Quantitative analysis of DAN content, nuclear morphmetric parameters and AgNOR count may be helpful in differentiating malignant and benign ameloblastomas.;