阿奇霉素体外对赫氏棘阿米巴的抑制作用

棘阿米巴是广泛存在于自然环境中、自由生活的致病性阿米巴,可引起人体棘阿米巴性角膜炎(acanthamoeba keratitis,AK)1],在艾滋病等免疫功能低下的人群中还会引起阿米巴性肉芽肿性脑炎、皮肤感染等2-3].棘阿米巴原虫包括滋养体和包囊两个阶段,当周围环境不利于生长或受到药物作用时,滋养体就转化为包囊而增强对环境和药物的抵抗能力.因此,临床上有关棘阿米巴感染的治疗效果不理想.阿奇霉素为新型半合成大环内酯类抗生素,临床上用于多种细菌、支原体和衣原体感染,但尚未见用于有关棘阿米巴感染的报道.我们通过体外研究观察阿奇霉素对棘阿米巴的杀伤作用.

In vitro inhibitory effect of azithromycin against Acanthamoeba healyi

Objective To observe the effect of azithromycin on Acanhtamoeba healyi (A.healyi) in vitro. Methods A.healyi was treated with 5.0 mg/ml, 50 mg/ml, 100 mg/ml of azithromycin respectively for 4 h, 8 h, 24 h, 48 h and 72 h in vitro and observed the growth status. A.healyi was also treated with 2 mg/ml of metronidazole as a control. Results The different concentrations of azithromycin did not show great inhibition effects to A.healyi compared to saline water and metronidazole at 4 h. The growth of A.healyi was inhibited when treated with 50 mg/ml, 100 mg/ml of the azithromycin for 6 h, and was significantly inhibited when treated with 100 mg/ml azithromycin at and after 8 h. Conclusion Azithromycin shows inhibitive effect on A.healyi in vitro and is superior to metronidazole.