Protocatechuic acid attenuates lipolysaccharide-induced acute lung injury |
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Authors: | Wei Miaomiao Chu Xiao Jiang Lanxiang Yang Xiaofeng Cai Qinren Zheng Chaochao Ci Xinxin Guan Mingfeng Liu Juxiang Deng Xuming |
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Institution: | (1) Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, People’s Republic of China;(2) Department of Dermatology, Second Hospital of Jilin University, Changchun, Jilin, 130041, People’s Republic of China;(3) College of Animal Science and Technology, Agriculture University of Hebei, Baoding, Hebei, 071000, People’s Republic of China |
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Abstract: | Protocatechuic acid (PCA) is a major metabolite of anthocyanins. It has numerous pharmacological effects, including anti-inflammatory,
antioxidant, and antitumoral activities. In the present study, we investigated the in vivo protective effect of PCA on acute lung injury (ALI) induced by lipolysaccharide (LPS) in mice. We treated mice with PCA 1 h
before the intratracheal (i.n.) administration of LPS. The pulmonary injury severity was evaluated 6 h after LPS administration.
We found that pretreatment with a 30 mg/kg of PCA markedly attenuated the LPS-induced histological alterations in the lung.
In addition, PCA inhibited the production of several inflammatory cytokines, including tumor necrosis factor alpha (TNF-α),
interleukin-1 beta (IL-1β), and IL-6, at 6 h in the bronchoalveolar lavage fluid (BALF) after LPS challenge. Furthermore,
PCA significantly reduced the number of total cells, neutrophils, and macrophages in the BALF, and it significantly decreased
the wet/dry weight (W/D) ratio of lungs and the protein concentration in the BALF. Additionally, Western blotting showed that
PCA efficiently blunted nuclear factor-kappa B (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα,
as well as the translocation of p65 from cytoplasm to the nucleus. In conclusion, these results indicate that PCA was highly
effective in inhibiting acute lung injury (ALI) and may be a promising potential therapeutic reagent for ALI treatment. PCA
may utilize the NF-κB pathway to attenuate the nonspecific pulmonary inflammation induced by LPS administration. |
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