Classical and molecular cytogenetics of <Emphasis Type="Italic">Khawia sinensis</Emphasis> (Cestoda: Caryophyllidea), invasive parasite of carp, <Emphasis Type="Italic">Cyprinus carpio</Emphasis> |
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Authors: | Martina Orosová Mikulá? Oros |
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Institution: | 1.Institute of Parasitology,Slovak Academy of Sciences,Ko?ice,Slovakia;2.Institute of Parasitology,Biology Centre ASCR,?eské Budějovice,Czech Republic |
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Abstract: | Chromosomes of the invasive tapeworm Khawia sinensis (Caryophyllidea), the specific parasite of common carp, were analyzed by means of conventional Giemsa staining and using
fluorescent DAPI and YOYO-1 dyes, silver staining, and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The karyotype
is composed of eight pairs of metacentric and telocentric chromosomes (2n = 16, n = 3m + 5t, TCL = 42.54 μm). Constitutive heterochromatin was located at pericentromeric regions of all pairs, except for the largest
metacentric pair (no. 1), which possessed no DAPI-positive band. FISH with rDNA probe revealed that both homologues of chromosome
pair no. 6 carry a cluster of ribosomal arrays, which were located interstitially close to the centromere. Present results
are compared with previous cytogenetic data on Khawia spp., and comments are made on the karyotypes with respect to their phylogenetic links. |
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