| HPLC-MS/MS多反应监测法测定榆黄蘑中甘露醇的含量 |
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| 引用本文： | 宋洋,孙佳明,王秀丽,张辉.HPLC-MS/MS多反应监测法测定榆黄蘑中甘露醇的含量[J].世界科学技术-中医药现代化,2008,10(1):85-87. |
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| 作者姓名： | 宋洋 孙佳明 王秀丽 张辉 |
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| 作者单位： | 长春中医药大学中医药与生物工程研发中心长春130117;长春中医药大学中医药与生物工程研发中心长春130117 |
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| 基金项目： | 吉林省教育厅科研基金项目( 2006229) : 北虫草的治疗标准研究, 负责人: 翁丽丽。 |
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| 摘 要： | 用MS/MS作为HPLC的检测器,采用多反应检测(MRM)扫描方式,建立了HPLC-MS/MS测定榆黄蘑中甘露醇含量的方法.该方法的线性范围为O.208-1.04mg·mL-1.通过样品的加标试验,回收率为97.12%,精密度RSD(n=5)为0.94%.实际样品分析表明,榆黄蘑中甘露醇的含量为38.9mg/g.本方法选择性高、灵敏、快速.也适用于其他复杂体系中甘露醇含量的快速测定.
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| 关 键 词： | 榆黄蘑 串联质谱 甘露醇 多反应监测 |
| 收稿时间： | 2007/9/13 0:00:00 |
| 修稿时间： | 2007/9/24 0:00:00 |
Measuring Mannitol in Pleurotus Citrinopileatus Using HPLC-MS/MS |
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| Song Yang,Sun Jiaming,Wang Xiuli and Zhang Hui.Measuring Mannitol in Pleurotus Citrinopileatus Using HPLC-MS/MS[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2008,10(1):85-87. |
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| Authors: | Song Yang Sun Jiaming Wang Xiuli and Zhang Hui |
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| Institution: | R&D Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese
Medicine, Changchun 130117;R&D Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese
Medicine, Changchun 130117 |
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| Abstract: | A method was developed to measure the mannitol in Pleurotus Citrinopileatus sing. Extracts, using HPLC-MS/MS and tandem mass spectrometer as a detector. Under an optimized condition, an effective linear range from 0.208 to 1.04 mg·mL- 1 was obtained, with a recovery of 97.12%, and a relative standard deviation of 0.94% (n=5). Analysis of real samples showed that the content of mannitol in Pleurotus Citrinopileatus sing. was 38.9mg/g. The proposed method is also able to make a quick measurement of manntiol in complex matrices. |
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| Keywords: | Pleurotus Citrinopileatus sing tandem mass spectrometry Mannitol multiple reaction monitoring |
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