| 中药DNA分子鉴定技术的发展与应用 |
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| 引用本文： | 徐红,王峥涛,胡之壁. 中药DNA分子鉴定技术的发展与应用[J]. 世界科学技术-中医药现代化, 2003, 5(2): 24-30 |
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| 作者姓名： | 徐红 王峥涛 胡之壁 |
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| 作者单位： | 上海中医药大学中药研究所 上海 200032;中国药科大学中药学院生药学研究室 南京 210038;上海中医药大学中药研究所 上海 200032 |
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| 基金项目： | 上海市科技发展基金项目(COOXD14022):中药石斛的活性成分与质量标准研究;;上海市重点科学(重中之中)建设项目;;上海市博士后基金项目:中药丹参的DNA分子鉴定与地道性研究 |
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| 摘 要： | 概述了近年来中药DNA分子鉴定技术在国内外的发展与应用。分析了RFLP、RAPD、DNA序列分析等常用技术的特点及在动、植物药材鉴定中的应用情况,展望了DNA分子鉴定技术在中药鉴定中的前景。
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| 关 键 词： | 中药； DNA分子鉴定； 技术； RFLP； RAPD； AP-PCR； |
| 收稿时间： | 2003-01-16 |
Development and Application of Technology for DNA Molecular Identification in Traditional Chinese Medicine |
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| Xu Hong an,Wang Zhengtao and Hu Zhibi. Development and Application of Technology for DNA Molecular Identification in Traditional Chinese Medicine[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2003, 5(2): 24-30 |
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| Authors: | Xu Hong an Wang Zhengtao Hu Zhibi |
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| Affiliation: | Institute of Chinese Materia Medica, Shanghai Univesity of Traditional Chinese Medicine, Shanghai 200032;Laboratory of Raw Pharmacognosy, College of Chinese Pharmacology, China University of Chinese Materia Medica, Nanjing 210038, Jiangsu Province, China;Institute of Chinese Materia Medica, Shanghai Univesity of Traditional Chinese Medicine, Shanghai 200032 |
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| Abstract: | This article generally states the development and application of the technology for DNA molecular identification to traditional Chinese medicine in China and abroad recent years analyzes the characteristics of the commonly-used techniques for sequential analysis, such as RFLP, RAPD and DNA and their application to the identification of medicinal animals and plants and looks forward to the prospects of this technology in the identification of Chinese medicines. |
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