| 利用LC／MS检测中药总成分中各大类成分含量的方法学探讨 |
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| 引用本文： | 李伯男,马超,王伟,邢东明,杜力军,肖盛元. 利用LC／MS检测中药总成分中各大类成分含量的方法学探讨[J]. 世界科学技术-中医药现代化, 2008, 10(4): 7-10 |
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| 作者姓名： | 李伯男 马超 王伟 邢东明 杜力军 肖盛元 |
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| 作者单位： | 清华大学生物科学与技术系药物药理研究室,北京100084;清华大学生物科学与技术系药物药理研究室,北京100084;清华大学生物科学与技术系药物药理研究室,北京100084;清华大学生物科学与技术系药物药理研究室,北京100084;清华大学生物科学与技术系药物药理研究室,北京100084;北京理工大学生命科学与技术学院,北京100081 |
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| 基金项目： | 清华大学科技开发项目(A2002162):感冒药YL2000的研究，负责人：杜力军; 博士学科点专项科研基金(20060003072):长期高剂量皮质酮对海马神经原胞内能量平衡的影响及淫羊藿苷的调节作用，负责人：杜力军; “十一”五国家科技支撑计划(2006BAI08B03-09):中药复方质量控制与评价方法研究，负责人：张启伟。 |
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| 摘 要： | 目的:利用液相-质谱(LC-Ms)技术检测总成分,并对其含量计算进行研究.方法:以感冒中药复方YL2000为例,利用LC-MS测定其中的总黄酮、总生物碱和总香豆素等主要大类成分,以HPLC测定的其中单体成分含量作参照,对LC-MS中各大类成分百分比进行含量计算.结果:以此方法计算的总黄酮、总生物碱、总香豆含量以及总含量与所用紫外分光光度法所测的含量基本一致.结论:利用LC-MS对成分结构的归属来确定各大类成分的构成比例,以所测代表成分HPLC含量结果作参照来计算各大类成分的含量具有一定的可行性.
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| 关 键 词： | 中药 总成分 质量标准 |
| 收稿时间： | 2008-04-21 |
| 修稿时间： | 2008-04-30 |
Methodology for Determining Major Components in Herbal Complex Using LC/MS/MS |
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| Li Bonan,Ma Chao,Wang Wei,Xing Dongming,Du Lijun and Xiao Shengyuan. Methodology for Determining Major Components in Herbal Complex Using LC/MS/MS[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2008, 10(4): 7-10 |
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| Authors: | Li Bonan Ma Chao Wang Wei Xing Dongming Du Lijun Xiao Shengyuan |
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| Affiliation: | Laboratory of Pharmaceutical Sciences; Department of Biological Sciences and Biotechnology; Tsinghua University; Beijing 100084;Laboratory of Pharmaceutical Sciences; Department of Biological Sciences and Biotechnology; Tsinghua University; Beijing 100084;Laboratory of Pharmaceutical Sciences; Department of Biological Sciences and Biotechnology; Tsinghua University; Beijing 100084;Laboratory of Pharmaceutical Sciences; Department of Biological Sciences and Biotechnology; Tsinghua University; Beijing 100084;Laboratory of Pharmaceutical Sciences; Department of Biological Sciences and Biotechnology; Tsinghua University; Beijing 100084;School of Life Science and Technology; Beijing Institute of Technology; Beijing 100081; China |
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| Abstract: | This paper examines the total elements of a Chinese complex(YL2000)using LC-MS.Based on the chemical structure(analogue),and using standard compounds as a reference,the total flavonoids,total alkaloids,and total coumarins have been determined.The quantity of three elements is similar to the quantity produced by the UV test. This is the first attempt to determine the total quantity of an element in a herbal complex,which may eventually be- come part of the TCM element quality control process. |
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| Keywords: | LC-MS Chinese medical complex total components quality control |
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