肺癌血管生成与淋巴结转移的关系

目的探讨肺癌血管生成与淋巴结转移的关系.方法我们采用免疫组织化学的方法检测了1997～1999年手术切除的50例肺癌病人石蜡标本中微血管密度和血管内生长因子及其受体蛋白的表达,其中有肺门淋巴结转移者24例,光镜下行MVD、VEGF、Flt、KDR蛋白表达细胞的计数.结果肺门淋巴结转移组的MVD、VEGF、Flt、KDR蛋白表达均高于未转移组.结论肺癌中MVD、VEGF、Flt、KDR蛋白表达与肿瘤转移行为密切相关,有可能作为判定肿瘤生物学行为、转移潜能及预后的指标.

The correlation between tumor angiogenesis and lymph node metastasis in lung cancer

Objective To study the relationship between tumor angiogenesis and lymph node metastasis in lung cancer. Methods Angiogenesis was assessed by the microvessel density(MVD),and expression of vascular endothelial growth factor(VEGF),fms-like tyrosine kinase(Flt),kinase insert domain-containing receptor(KDR) using immunohistochemical staining.Paraffin-embedded specimens from 50 patients with lung cancer who underwent mastectomy from 1994 to 1995 were studied.Twenty-four patiens had histologically proved positive lung lymph node, microvesels and VEGF,Flt,KDR positive stained cancer cells or endothelial cells were counted with light microscope.Results The result showed the protein expression higher in the group of lymph node positive than that in the group of lymph node negative. Conclusion So we thought that MVD,and the expression of VEGF,Flt,KDR were highly correlation with metastasis in esophageal cancer ,which may serve as a parameter for determining tumor biological,metastatic potential and prognosis.