CpG ODN对ZP~(121-140)表位合成肽诱导的免疫应答和免疫避孕效应影响

目的:探讨CpG ODN对透明带(四)抗原表位ZP~(121-140)合成肽诱导的免疫应答和免疫避孕效应影响.方法:应用人工合成ZP2~(121-140)表位肽,与20μg CpG ODN或等量完全弗氏佐剂(CFA)混悬后左胫前肌免疫雌性BALB/c小鼠,初次免疫后2、4、6周各加强免疫一次,共免疫四次.在每次加强免疫前和末次免疫后两周断尾取血,分离血清,分析特异性lgG抗体及非特异性细胞因子IFN-γ、TNF-Ⅱ,IL-10水平;收集小鼠阴道冲洗液,离心取上清,分析特异性IgA抗体水平.免疫小鼠生育实验结束后,取卵巢组织行病理学分析.结果:CpG ODN组诱导的特异性IgG和IgA水平较CFA组有增高趋势,但无显著性差异(P＞O.05).CpG ODN组诱导的非特异性IFN-γ和TNF-水平明显高于CFA组(P＜0.05);而CpG ODN组诱导的IL-10水平显著低于CFA 组(P＜0.05).两种佐剂对小鼠的受孕率影响没有显著性差异,但CpG ODN组孕鼠每胎产仔数明显低于CFA组(P＜O.05).病理学分析显示实验小鼠卵巢组织无病理性变化.结论:CpG ODN对Zp~(121-140)合成肽诱导的免疫应答和免疫避孕效应略优于CFA,更适合用于避孕疫苗佐剂研究.

The effect of CpG ODN on the immune responses and immune-contraception induced by ZP~(121-140) synthetic peptide

Objective:To investigate the effect of CpG ODN on the immune responses and immune-contraception induced by ZP~(121-140) synthetic peptide.Methods: BALB/e mice were given an injection into the left tibialis anterior muscle of ZP~(121-140) synthetic peptide with 20 μg CpG ODN or CFA,then the mice were given other injections at 2,4,6 weeks using the same formulation.The mice' s blood was collected before each vaccination and after the last vaccination every 2 weeks.The specific IgG and IgA in sere and non-specific cytokines IFN-γ,TNF-α,IL-10 in vaginal mucosa were measured by ELISA.The ovarial pathological changes were undertaken using hemattxylin and eosin-stained paraffin sections.Results:The specific IgG in sera and IgA in vaginal mucosa induced by ZP~(121-140) synthetic peptide combined with CpG ODN were no more than those of ZP~(121-140) synthetic peptide combined with CFA.There were significant increases in IFN-γ,and TNF-α when CpG ODN was mixed with ZP121-140 synthetic peptide and the increase of CpG ODN was more significant than that of CFA.Otherwise there was a significant decrease in IL-10 when CpG ODN was mixed with ZP~(121-140) synthetic peptide and the decrease of CpG ODN was more significant than thai of CFA.There was no significant difference in the rate of pregnancy between CpG ODN group and CFA group,but the average number of birth mice in CpG ODN group was less than that in CFA group.No pathological changes were found in the ovaries of experimental mice.Conclusion: The adjuvant effect of CpG ODN is more advantageous than that of CFA in contraception vaccine research.