鲨鱼肝提取液的免疫活性实验研究

从鲨鱼肝脏中提取一组能有效促进成纤维细胞等多种组织细胞增殖、分化的小分子活性多肽，体外探讨其生物活性及其对人细胞免疫功能的影响。方法：分别用~３Ｈ－ＴｄＲ ＤＮＡ掺入法、细胞学技术、ＥＬＩＳＡ、生物素－链霉亲和素（ＢＳＡ）免疫细胞化学法及~（１２５）Ｉ－ＵｄＲ释放试验检测其免疫活性。结果：可明显增强肝瘤细胞株７２１１细胞ＤＮＡ合成，有效提高Ｔ淋巴细胞形成Ｅａ花环能力（Ｐ＜０．０１），诱导ＰＢＭＣ细胞分泌ＩＦＮ－γ（Ｐ＜０．０１），增强ＮＫ细胞活性（Ｐ＜０．０５），促进ＰＢＭＣ细胞表面ＨＬＡ－ＤＲ抗原的表达（Ｐ＜０．００１）。结论：鲨鱼肝提取液对机体细胞免疫功能具有良好的正向调节作用，提示其在改善机体免疫状态，提高机体免疫防御机制等方面具有较高的应用价值。

Study on Immune Activity of Shark Liver Extracts

Purpose: A group of ploypeptides that can effectively promote the proliferation and differentiation of many cells (for example, fibroblasts etc. ) were extracted from shark liver, and its biologic activity and the effect on human cellular immunity function in vitro was investigated. Methods: Immunocompetence of shark liver extracts were measured respectively by 3H-TdR intermingle assay, cellular technique, ELISA, biotin-streptomycin labeled avidin (BSA) assay and 125I-UdR release test. Results: Shark liver extracts could significantly accelerate DNA synthysis of 7211 cells, enhance Ea- rossette forming cell rate of human peripheral blood mononuclear cells (PBMC) (P<0. 01), induce the secretion of IFN-γ by PBMc (P<0. 01), increase the activity of NK cells (P< 0. 05), and boost the expression of HLA-DR antigens (P<0. 001). Conclusion: Shark liver extracts can adjust positively the human cellular immunity. The results implied that it ha the higher values for application and research on approving immunologic conditions and enhancing homunologic defence.