| 人尿中三种不同分子量的蛋白质对几种癌细胞的抑制作用 |
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| 引用本文： | 傅更锋 陈钧辉. 人尿中三种不同分子量的蛋白质对几种癌细胞的抑制作用[J]. 中国生化药物杂志, 1999, 20(1): 7-9 |
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| 作者姓名： | 傅更锋 陈钧辉 |
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| 作者单位： | 南京大学生物化学系医药生物技术国家重点实验室!210093 |
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| 基金项目： | 江苏省自然科学基金!（BK 97036），南京大学现代分析中心测试基金 |
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| 摘 要： | 以新鲜的正常男性尿液为原料，经分离、纯化和鉴定出其分子量分别为 ２８、３５和 ５０ ｋＤ的三种蛋白质混合物，命名为Ｕ－１。采用体外试验的方法，测定了Ｕ－１对三种人癌细胞的增殖和ＤＮＡ合成的抑制作用。结果表明在低血清培养体系中对人肝癌细胞（７７２１）、人白血病细胞（Ｕ_(９３７)）和人胃癌细胞(ＭＫＮ－４５)的增殖和ＤＮＡ合成均最大抑制作用，并有很好的剂－效关系．当Ｕ－１在培养液中的浓度为０．２４ｍｇ／ｍｌ时，就能产生明显的抑制作用，达到最大抑制作用一半时 Ｕ－１的浓度（ＬＤ５０）为 ０． ２４～２ ．４ ｍｇ／ｍｌ。
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| 关 键 词： | 人尿 抗癌物质 细胞计数 ~3H-胸腺嘧啶核苷参入 |
Inhibition of Three Proteins of Different Molecular Weight Isolated from Human Urine on the Growth of Several Tumor Cells |
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| Fu Gengfeng,Chen Junhui,Wang Xinchang. Inhibition of Three Proteins of Different Molecular Weight Isolated from Human Urine on the Growth of Several Tumor Cells[J]. Chinese Journal of Biochemical Pharmaceutics, 1999, 20(1): 7-9 |
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| Authors: | Fu Gengfeng Chen Junhui Wang Xinchang |
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| Abstract: | The mixture of three proteins, named U-1, are isolated from normal man's urine, their molecular weight are 28, 35 and 50 kD. In vitro, U-1 can inhibit the proliferation and DNA replication of three human cancer cells. U-1 can inhibit human heptomic cell (7721 ), human Leukemic cell (U937 ) and human stomachic cell (MKN-45) in cultral liquid of low serum. The inhibition will be reinforced with the dose increasing. When the concentration of U-1 is 0. 24 mg/ml, it has significant inhibition to cancer cells, the LD50 is 0. 24-2.4 mg/ml. The action of anticancer of U-1 has something to do with the nature of antitumor in human urine. |
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| Keywords: | Human urine Anticancer substance Cell counting Incorporation of ~3H-TdR |
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