| ATP对原代培养大鼠膀胱Cajal间质细胞及逼尿肌细胞兴奋性的影响 |
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| 引用本文： | 樊汝会,王永权,李雪梅,李为兵,宋波,周占松,方强.ATP对原代培养大鼠膀胱Cajal间质细胞及逼尿肌细胞兴奋性的影响[J].中国局解手术学杂志,2011,20(2):117-119. |
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| 作者姓名： | 樊汝会 王永权 李雪梅 李为兵 宋波 周占松 方强 |
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| 作者单位： | 第三军医大学西南医院全军泌尿外科研究所,重庆,400038 |
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| 摘 要： | 目的探讨外源性ATP对大鼠膀胱Cajal间质细胞（ICC）兴奋性的影响。方法采用胶原酶消化法急性分离SD大鼠膀胱ICC和逼尿肌细胞（DSMC）,通过激光共聚焦技术检测外源性ATP作用前后ICC及DSMC内Ca2＋信号的变化。结果胶原酶P消化后,在干细胞因子（SCF）的刺激下,经c-k it免疫荧光染色阳性的ICC呈长梭形或纺锤形,胞体较小,并且带有分枝状突起,而DSMC呈纤维形或短棒状,二者形态有明显区别。F luo-4 NW染色后,激光共聚焦显微镜观察显示,静止状态下ICC内Ca2＋]i平均荧光强度（55.16±3.23）显著强于平滑肌细胞（45.59±2.08）,P〈0.05;20μmol/L和100μmol/L ATP可诱发体外培养的ICC及DSMC细胞内Ca2＋]i一过性升高,随后缓慢下降。结论在SCF刺激条件下,SD大鼠膀胱ICC和DSMC可在体外稳定混合培养。外源性ATP可兴奋体外混合培养的ICC和DSMC。
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| 关 键 词： | 膀胱 ATP Cajal间质细胞 兴奋性 |
Effect of ATP on excitability of interstitial cells of Cajal and detrusor smooth muscle cell in primary cultured rat bladder |
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| FAN Ru-hui,WANG Yong-quan,LI Xue-mei,LI Wei-bing,SONG Bo,ZHOU Zhan-song,FANG Qiang.Effect of ATP on excitability of interstitial cells of Cajal and detrusor smooth muscle cell in primary cultured rat bladder[J].Journal of Regional Anatomy and Operative Surgery,2011,20(2):117-119. |
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| Authors: | FAN Ru-hui WANG Yong-quan LI Xue-mei LI Wei-bing SONG Bo ZHOU Zhan-song FANG Qiang |
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| Institution: | FAN Ru-hui,WANG Yong-quan,LI Xue-mei,LI Wei-bing,SONG Bo,ZHOU Zhan-song,FANG Qiang(Urological Research Institute of PLA,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To explore the effect of ATP on excitability of interstitial cells of Cajal(ICC) and detrusor smooth muscle cells(DSMC) in SD rat bladder.Methods ICC and DSMC in SD rat bladder were isolated by collagenase digestion and the effects of ATP on variation of Ca2+ in ICC and DSMC were assessed by confocal laser scanning microscopy(CLSM).Results Obvious morphological differences were observed between ICC and DSMC.The positive ICC were long shuttle-shaped or spindle-shaped,small with branch-like protrusi... |
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| Keywords: | ATP |
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