肺炎支原体两种检测方法的比对分析

目的：探讨肺炎支原体（MP）咽拭子聚合酶链反应（PCR）法和血清间接免疫荧光法（IIFA）两种检测方法的不同。方法：将198例疑似MP感染的患儿根据年龄分为两组：A组（0~3岁，n=75）、B组（3~12岁， n=123），分别取咽拭子用PCR法测MP-DNA，取血清用IIFA法测MP-IgM，比较两种方法的阳性率。结果：MP-DNA检测阳性率为57.07%，MP-IgM检测阳性率为47.47%，两者比较差异有统计学意义（P〈0.01）。A组MP-DNA阳性率48.00%明显高于同组MP-IgM阳性率25.33%，差异有统计学意义（P〈0.01）；B组MP-DNA和MP-IgM阳性率分别为62.60%、60.98%比较差异无统计学意义（P〉0.05）。结论：PCR与IIFA联合检测可以提高MP感染诊断的准确性。

Comparison of Two Diagnostic Methods for Detecting Mycoplasma Pneumoniae

Objective：To compare the differences between polymerase chain reaction（PCR）and indirect immunofluorescence（IIFA）for detecting the Mycoplasma pneumoniae（MP）.Method：A total of 198 children with suspected MP infection were divided into group A（0-3 years old,n=75）and group B（3-12 years old,n=123）according to their ages.Oropharyngeal swabs and blood were collected from the children,MP-DNA was detected by PCR and MP-IgM was detected by IIFA separately.The positive rate of two methods were compared.Result：The positive rate of MP-DNA detection and MP-IgM were 57.07%,47.47%,the difference was statistically significant（P〈0.01）.In group A the positivity of MP-DNA（48.00%）was significantly higher than the MP-IgM（25.33%）,the difference was statistically significant（P〈0.01）, and there was no significant difference of MP-DNA（62.60%）and MP-IgM（60.98%）in group B（P〉0.05）.Conclusion：The joint detection of PCR and IIFA can improve MP detecting accuracy.