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精氨酸类似物对一氧化氮合酶的影响
引用本文:孙飞,吴赛珠,张晓添,刘长青. 精氨酸类似物对一氧化氮合酶的影响[J]. 南方医科大学学报, 2006, 26(10): 1431-1433
作者姓名:孙飞  吴赛珠  张晓添  刘长青
作者单位:1. 南方医科大学南方医院心内科,广东,广州,510515
2. 解放军大连第一疗养院药剂科,辽宁,大连,116023
基金项目:广东省广州市科技攻关项目
摘    要:目的 研究新精氨酸类似物对一氧化氮及诱导型一氧化氮合酶(iNOS)的影响。方法 脂多糖刺激巨噬细胞,检测不同浓度新精氨酸类似物对诱导型iNOS的抑制程度;胰岛素刺激脐静脉内皮细胞,检测新精氨酸类似物对于内皮细胞的iNOS的抑制作用。结果 (1)新精氨酸类似物可显著抑制脂多糖刺激的巨噬细胞产生一氧化氮,并呈剂量依赖性。而对胰岛素刺激的内皮细胞一氧化氮产生无明显影响。(2)新精氨酸类似物可显著降低巨噬细胞iNOS的活性,对于巨噬细胞的结构型iNOS活性及内皮细胞的iNOS活性无明显影响。(3)新精氨酸类似物对于巨噬细胞iNOS的蛋白表达无明显影响。结论 新精氨酸类似物是一种具有高度选择性的iNOS抑制剂。

关 键 词:精氨酸类似物  一氧化氮  一氧化氮合酶
文章编号:1673-4254(2006)10-1431-03
收稿时间:2005-12-06
修稿时间:2005-12-06

Highly selective inhibition of inducible nitric oxide synthase in vitro by a tripeptide as a new arginine analog
SUN Fei,WU Sai-zhu,ZHANG Xiao-tian,LIU Chang-qing. Highly selective inhibition of inducible nitric oxide synthase in vitro by a tripeptide as a new arginine analog[J]. Journal of Southern Medical University, 2006, 26(10): 1431-1433
Authors:SUN Fei  WU Sai-zhu  ZHANG Xiao-tian  LIU Chang-qing
Affiliation:Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. sunfei@fimmu.com
Abstract:Objective To investigate the inhibitory effect of a tripeptide, a new arginine analog, on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS). Methods Macrophages challenged with lipopolysaccharide were cultured to test the inhibitory effect of the arginine analog of different concentrations on iNOS. Primarily cultured human umbilical vein endothelial cells (HUVECs) treated with insulin were used to test the effect of the analog on endothelial NOS (eNOS). Results The new arginine analog significantly inhibited NO production in the macrophages in a dose-dependent manner, but had little effect on insulin-stimulated NO production in HUVECs. The new analog could significantly suppress iNOS activity, but had no significant inhibitory effect on constitutive NOS activity or eNOS activity. Western blot analysis showed that the new analog did not significantly affect the protein expression of iNOS. Conclusion The new analog is a NOS inhibitor with high selectivity on iNOS.
Keywords:arginine analog   nitric oxide   nitric oxide synthase
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