宫颈癌染色体杂合性缺失和微卫星不稳定性分析

目的：分析宫颈癌染色体部分位点杂合性缺失（LOH）及微卫星不稳定性（MSI）,探讨其与宫颈癌的关系。方法：选取6个微卫星位点,采用PCR、变性聚丙烯酰胺凝胶电泳及硝酸银染色对59例宫颈癌和49例宫颈上皮内瘤变（CIN）标本进行检测。结果：所有位点的CIN和浸润癌之间的MSI无统计学差异,而D3S1234、D3S1300和D3S1289位点在低级别CIN、高级别CIN和浸润癌三者之间的LOH差异有统计学意义。结论：染色体3p区域的LOH是宫颈癌中的早期事件,而染色体4p区域的LOH在宫颈癌早、晚期均可见到,因此,联合检测多个位点的LOH对于宫颈癌的早期诊治及判断预后有重要意义。

Analysis of loss of heterozygosity and microsatellite instability of chromosome in human cervical carcinoma

Objective：To analyze the loss of heterozygosity （LOH） and microsatellite instability （MSI） at partial sites of chromosome in human cervical carcinoma, and investigate their roles in the disease. Methods： six sites of microsatellites were selected. LOH and MSI were detected with polymerase chain reaction （PCR） and denaturing polyacrylamide gel electrophoresis in 59 cases of cervical carcinoma and 49 cases of cervical intraepithelial neoplasia （CIN）. Results： No significant difference was observed in MSI rate between cervical carcinoma and CIN at all the sites. There was significant difference in LOH rate among lower level CIN, higher level C IN and cervical carcinoma at D3S1234, D3S1300 and D3S1289. Conclusion：The 3p LOH is an early event in cervical carcinoma. The 4p LOH happened at early and late stage of tumor progression. The detection of LOH rate at multi - sites plays an important role in the early diagnosis, treatment and estimation of prognosis in cervical carcinoma.