反相斑点杂交快速诊断解脲脲原体基因型及其临床意义

目的:建立1个能用于临床标本直接分型的简单、实用、可靠性好的解脲脲原体基因分型方法;揭示正常携带状态及感染状态下解脲脲原体的基因型。方法:设计分型引物,用反相斑点杂交进行解脲脲原体基因型鉴定。结果:病例组与对照组解脲脲原体基因型的检出率差异无统计学意义,总检出率为89.1%。对照组中解脲脲原体以parvum群(微小脲原体)为主(79.3%),其检出率较女性下生殖道感染患者高(P=0.018),而且以其中的1、3、6基因型的单型别为主(82.6%)。病例组解脲脲原体中两群感染(16.2%)较对照组高(6.9%),差异有统计学意义(P=0.033),其中以T960群与1型的多型别感染在病例组较对照组高(P=0.009);在多型别感染中1型的检出率病例组较对照组高(P=0.026),而单型别感染中1型的检出率在对照组高(P=0.000)。结论:反相斑点杂交能进行解脲脲原体基因分型,方法简单、实用,有望发展成为用于临床标本的解脲脲原体分型的1种方法。Parvum群(微小脲原体),尤其是其中的1、3、6的单型别是正常人群携带的可能性较大。女性下生殖道感染患者解脲脲原体的两群感染较正常体检人群高,尤以T960群与parvum群中1型的多型别感染较正常体检人群高,提示T960群有可能和1型起协同作用或独自导致疾病。

Rapid diagnosis the genotype of Ureaplasma urealyticum by Reverse dot blot and clinical significance

Objective:To establish Reverse dob blot hybridization for rapid derection of genotypes of Ureaplasma urealyticum,to assess its value in clinical applications.Another aim of this study was determine the distribution of different Ureaplasma urealyticum genotypes among physical check-up women and the gential tract infection patients.Methods:Primers for typing were designed and detect genotype of Ureaplasma urealyticum by Reverse dot blot hybridization(RDB).Results:The detection rate of genotype of Ureaplasma urealyticum were no significant difference between the above of two groups,Total detection rate of two groups was 89.1%.Infection with only one genotype(genotype 1,3 or 6) of U.parvum is frequently found in control group;Two-biovar infection and T960 biovar mixed with genotype 1 are more prevalent in patients in case group than in control group women(P=0.033 and P=0.009,respectively).Conclusion:The RDB method described here is relatively simple,rapid for biotyping between U.parvum and U.urealyticum and genotyping of genotypes 1,3,6,and 14 in U.parvum.It may be used as a tool in rapid diagnosing for genotype of Ureaplasma urealyticum.People Infected with only one genotype(genotype 1,3 or 6) of U.parvum may be normal carriers.Two-biovar infection and T960 biovar mixed with genotype 1 are more prevalent in patients with the genital infection than in normal women taking medical examination.T960 biovars mixed with genotype 1 may be more frequently associated with the genitial tract infection.