卵巢癌CYP1A1基因的MspI位点多态性分析

目的：检测卵巢癌患者CYP1A1基因的 MspI位点多态性，探讨CYP1A1基因MspI位点多 态性与患者临床资料的关联。方法：术后病理证实为卵巢恶性肿瘤患者81例，抽取手术前空腹外周血，进行CYP1A1的MspI位点多态性检测；对81例不同基因型卵巢癌 患者相应的临床资料进行比较。结果：81例卵巢癌患者CYP1A1基因中，Ａ基因型（野生型T/T）47例（58％），Ｂ基因型(杂合型T/C) 25例（31％），Ｃ基因型(突变纯合型C/C)9例（11％）。12～29岁患者基因型频数分布为A基因型1例（2.1%），B基因型5例(20.0%)，C基因型0例； 30～49岁患者基因型频数分布为A基因型12例（25.5%），B基因型8例（32.0%），C基因型3例（33.3%）；50～69岁患者基因型频数分布为A基因型31例（66.6%），B基因型8例（32.0%），C基因型4例（44.4%）；≥70岁患者基因型频数分布为A基因型3例（6.4%），B基因型4例（16.0%），C基因型2例（22.2%）。各基因型患者的发病年龄比较差异均具有显著性(P<0.05)。上皮性肿瘤A基因型40例（85.1%），B基因型15例（60.0%），C基因型9例（100%），3种基因型卵巢病理类型的构成比比较差异具有显著性（P<0.05）。结论 ：卵巢癌患者存在CYP1A1基因MspI多态性；卵巢癌的发病年龄与CYP1A1基因型有关；卵巢上皮 性恶性肿瘤发生率A基因型（野生型T/T）高于其他基因型；携带突变等位基因C的患者卵巢非上皮性肿瘤的比例呈增加趋势。

Polymorphism analysis of CYP1A1 MspI in ovarian cancer

Objective To detect the polymorphism of CYP1A1-MspI gene in patients with ovarian cancer.and discuss the relationship between the polymorphism ofCYP1A1-MspI gene and correspond cases' general materials and clinical materials.Methods The free peripheral blood samples of 81 cases confirmed to be ovarian cancer by postoperative pathology were collected preoperatively and the polymorphism of CYP1A1-MspI gene was detected.The clinical materials of the 81 cases with different genotypes were compared.The relationship between the polymorphism and clinical materials was analyzed.Results Among the 81 cases of ovarian cancer,there were 47 cases of wild type-genotype A(T/T)(58%),25 cases of mutation heterozygosis-genotype B(T/C)(31%),and 9 cases of mutation homozygosis-genotype C(C/C)(11%).The genotypic frequency distribution in patients aged from 12 to 29 was one case of genotype A(2.1%),5 cases of genotype B(20.0%),and no case of genotype C.The genotypic frequency distribution in patients aged from 30 to 49 was 12 case of genotype A(25.5%),8 cases of genotype B(32.0%),and 3 cases of genotype C(33.3%).The genotypic frequency distribution in patients aged from 50 to 69 was 31 case of genotype A(66.0%),8 cases of genotype B(32.0%) and 4 cases of genotype C(44.4%).The genotypic frequency distribution in patients aged more than 70 years was 3 case were of genotype A(6.4%),4 cases of genotype B(16.0%),2 case of genotype C(22.2%).There were significant differences of the ages of onset between patients with different CYP1A1-MspI genotypes (P<0.05).There were 40 cases of genotype A(85.1%),15 cases of genotype B(60.0%),and 9 cases of genotype C(100%) in epithelial cancer patients.There significant differences of the constituent ratio of the ovarian cancer pathological types between patients with different genotypes(P<0.05).Conclusion Polymorphism of CYP1A1-MspI gene exisits in patients with ovarian cancer.The age of onset of patients with ovarian cancer is related with CYP1A1-MSPI genotypes;The incidence of epithelium tumor in patients with genotype A is higher than that of other ones.The ratio of non-epithelial tumor in the patients with mutation allele gene C has an increasing tendency.