| 三氧化二砷对成纤维细胞的生长抑制及细胞周期的影响 |
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| 引用本文： | 肖莉,杨丽丽. 三氧化二砷对成纤维细胞的生长抑制及细胞周期的影响[J]. 实用药物与临床, 2010, 13(5) |
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| 作者姓名： | 肖莉 杨丽丽 |
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| 作者单位： | 1. 中国医科大学附属盛京医院,急诊科,沈阳,110004
2. 中国医科大学附属盛京医院,风湿免疫科,沈阳,110004 |
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| 基金项目： | 沈阳市科技局科学计划基金资助，辽宁省博士启动基金 |
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| 摘 要： | 目的探讨三氧化二砷(AS2O3)对成纤维细胞的增殖及细胞周期的影响。方法用含AS2O3浓度分别为0、2、4μmol/L的培养液分别作用于成纤维细胞,24 h和48 h后,MTT法检测细胞增殖;流式细胞术(FCM)检测细胞周期分布。结果 AS2O3可显著抑制成纤维细胞增殖,并呈时间和剂量依赖性(P<0.01);AS2O3可使细胞周期G0/G1期比例增加(P<0.01),G2/M期减少(P<0.01)。结论 AS2O3具有抑制成纤维细胞的增殖及阻止细胞周期进展的作用。
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| 关 键 词： | 三氧化二砷 成纤维细胞 细胞增殖 细胞周期 |
Effects of arsenic trioxide on cell proliferation and cell cycle of 3T3 fibroblast line |
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| XIAO Li,YANG Li-li. Effects of arsenic trioxide on cell proliferation and cell cycle of 3T3 fibroblast line[J]. Practical Pharmacy and Clinical Remedies, 2010, 13(5) |
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| Authors: | XIAO Li YANG Li-li |
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| Abstract: | Objective To investigate the effects of arsenic trioxide(AS2O3)on proliferation and cell cycle of 3T3 fibroblast line.Methods 3T3 fibroblasts were cultured in standard medium as control group or medium supplemented with 2 μmol/L,4μmol/L of AS2O3 for 24 h and 48 h respectively.MTT assay was carried out to study cell proliferation and cell cycle was detected by flow cytometry(FCM).Results AS2O3 could significantly inhibit the proliferation of fibroblasts time-dependently and dose-dependently(P<0.01);G0/G1 phase ratios increased and G2/M phase ratios decreased significantly in a dose-dependent manner.Conclusion AS2O3 could inhibit fibroblast proliferation and prevent cell cycle progression. |
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| Keywords: | Arsenic trioxide Fibroblast Cell proliferation Cell cycle |
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