成年大鼠脑创伤后脑组织碱性成纤维细胞生长因子表达的变化

目的:研究液压冲击性脑损伤后成年大鼠脑组织碱性成纤维细胞生长因子(bFGF)表达时程和空间分布的变化。方法:制作液压冲击性脑损伤模型,免疫组织化学法动态检测内源性bFGF的变化。结果:在正常脑组织中,bFGF免疫活性低水平表达,且主要位于胶质细胞核及神经元细胞质中。液压冲击伤后bFGF免疫活性增强,聚集核周边。此外,部分bFGF免疫活性聚集于神经元周围间隙中半定量分析显示液压冲击伤后3d,大脑皮质bFGF阳性细胞数量较正常对照组升高4.8倍;伤后7 d,bFGF免疫活性达高峰,伤后30 d消失。结论:液压冲击伤后,损伤的胶质细胞和神经元暂短地合成bFGF,并以旁分泌和自分泌形式起作用。

Changes in Spatial and Temporal Expression of Basic Fibroblast Growth Factor in Adult Rat Brain Following Fluid Percussion Injury in Vivo

Aim:To investigate changes in spatial and temporal expression of basic fibroblast growth factor (bFGF) in the adult rat brain following fluid percussion injury (FPI). Methods: The lateral fluid persussion brain injury models were made and the dynamic expressions of bFGF were determined by immunocytochemistry. Results: In the control group, the localization of limited bFGF immunoreactivity in cells was the nuclei of glial cells and cytoplasm of neurons. After FPI, bFGF immunoreactivity was intensified and located perinuclearly. In addition, increased diffuse bFGF immunoreactivity was intensified and located perinuclearly. In addition, increased diffuse bFGF immunoreactivity was located surrounding neuronal cell bodies. Semiquatitative analysis showed that the number of bFGF-positive cells in the cerebral cortex increased approximately 4.8-fold at 3 days postinjury; bFGF immunoreactivity was strikingly increased to the greatest extent at 7 days, disappeared by 30 days after FPI. Conclusion: The results demonstrate that the injuried glia cells and neurons synthesized transiently a large amount of bFGF, which might act as a paracrine/autocrine factor.