TNF—α、IL—8在机械通气致肺损伤中的作用

目的探讨肿瘤坏死因子α(TNF-α)和白细胞介素8(IL-8)在机械通气致肺损伤中的可能作用。方法24只普通健康小猪随机等分为对照组、低潮气量组(A组)、正常潮气量组(B组)、大潮气量组(C组)，采用持续给予小猪不同潮气量通气动物模型，利用放射免疫法、酶联免疫法(ELISA)和髓过氧化物酶(MPO)测定法，分别检测不同潮气量组通气1、3、7d后，血清和肺组织匀浆中TNF-α、IL-8及MPO含量的变化。结果A、B、C组血清、肺组织匀浆TNF-α、IL-8及MPO的含量较对照组升高(P＜0.05或P＜0.01)，其中3d后，TNF-α达峰值；7d后IL-8及MPO达峰值，以A、C组明显。结论TNF-α、IL-8在机械通气致肺损伤中可能发挥重要作用。

Role of TNF-

Objective　Investigate the possible roles of tumor necrosis factor alpha(TNF-α)，interlukin-8(IL-8)in the lung injury induced by mechanical ventilation.Methods　Twenty-four normal healthy small pigs were given continuous different tidal volume as animal model,they were random divided into control group,low tidal volume group(group A),normal tidal volume group(group B),and high tidal volume group (group C), contents of TNF-α、IL-8 and myeloperoxidease (MPO) in serum and lung were observed with radioimmumoassay and enzyme linked immunosorbent assay and MPO measurent 1 d,3 d and 7 d following ventilation respectively.Results　The contents of TNF-α、IL-8 and MPO in serum and lung were higher in group A、B and C than those in control group(P＜0.05 or P＜0.01).In addition,TNF-a value was at it's maximum 3 d later,IL-8 value and MPO value were at their maximum 7 d later,above change were significantly in group A and group C.Conclusion　TNF-α、IL-8 may play important roles in the lung injury induced by mechanical ventilation.