肝脏移植后的免疫损伤与热休克蛋白的表达

目的 探讨肝脏移植后的免疫损伤与几种主要分子伴侣(热休克蛋白)表达状况之间的内在规律。方法 34份移植后肝脏穿刺标本和10份正常肝脏标本，分为A(无排斥反应)组、B(轻／中度急性排斥反应)组、C(重度急性排斥反应)组、D(慢性排斥反应／肝纤维化)组、E(对照)组。进行HSP60、HSP70、HSP90、HO—1等四种分子伴侣免疫组织化学分析和图像分析。结果 B和C组各指标间无统计学差异，A、D、E与B、C组间有统计学差异。HSP60在移植肝脏中表达降低，排斥反应发生时高；HSP70和HSP90在移植肝脏中升高。HO—1肝脏移植后升高。结论 不同种类的热休克蛋白依据自身表达的特点对移植后的免疫损伤作出反应，体现了细胞的自我保护机制。

Relationship between immune injury and expression of heat shock protein after liver transplantation

Objective To investigate the relationship between immune injury and expression of molecular chaperone (heat shock protein) after liver transplantation. Methods Thirty-four punctured samples of donor liver after transplantation and 10 samples of normal liver were divided into group A (no rejection), group B (mild/moderate acute rejection), group C (serious acute rejection), group D (chronic rejection/fibrosis) and group E (the controls). The molecular chaperones of HSP60, HSP70, HSP90 and HO-1 were analyzed with immunohistochemistry and imaging. Results There was no significant difference between group B and group C. There were marked differences between groups A, D and E and groups B and C. The expression of HSP60 was lower in donor liver than in the normal liver but became high when acute rejection occurred. The expression of HSP70 and HSP90 was higher in donor liver than in the normal one. Expression of HO-1 became higher after transplantation. Conclusions Different molecular chaperones respond to immune injury according to their own characteristics after liver transplantation, which shows the mechanism of cellular self-protection.