| 妇康洗剂质量标准研究 |
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| 引用本文： | 谈静,方芳,曾倩,等. 妇康洗剂质量标准研究[J]. 中药与临床, 2014, 0(2): 48-52 |
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| 作者姓名： | 谈静 方芳 曾倩 等 |
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| 作者单位： | 成都中医药大学附属医院;成都中医药大学; |
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| 基金项目： | 成都中医药大学附属医院基金项目(2009-D-YY-44) |
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| 摘 要： | 目的:建立妇康洗剂的质量标准。方法:采用TLC法对处方中苦参、紫草、甘草、乌梅进行定性鉴别;采用HPLC法以乙腈-甲醇-0.1%磷酸溶液(三乙胺调pH至7.5)(12:5:83)为流动相;在检测波长220 nm处测定氧化苦参碱和苦参碱的含量。结果:TLC鉴别分离度良好,专属性强。氧化苦参碱在0.1946~2.9190μg,相关系数r为0.9999,线性关系良好;苦参碱在0.1134~1.7010μg,相关系数为0.9999,线性关系良好。平均加样回收率分别为99.63%(RSD为2.19%)、99.12%(RSD为1.33%)。结论:该方法简便、准确,重复性好,可有效控制妇康洗剂的质量。
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| 关 键 词： | 妇康洗剂 薄层鉴别 高效液相色谱 氧化苦参碱 苦参碱 质量标准 |
The quality standards research of Fukang lotion |
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| Affiliation: | TAN Jing, FANG Fang, ZENG Qian, YUAN Qiang-hua(1.Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan; 2. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan) |
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| Abstract: | Objective：To establish the quality standards of Fukang lotion. Method：TLC was used to identify Kushen, Zicao, Gancao and Wumei in Fukang lotion. The content of oxymatrine and matrine were determined by HPLC with a mobile phase of acetonitrile-Methano1-0.1% phosphoric acid （12：5：83）. And Triethylamine was used to maintain pH value at 7.5.The detection wave length was set at 220 nm. Result：The identification by TLC was distinct and highly specific. The linear ranges of oxymatrine and matrine were in the ranges of 0.1946μg -2.9190μg （r=0.9999） and 0.1134μg -1.7010μg （r=0.9999）. The sample recoveries were 99.63% （RSD=2.19%） and 99.12% （RSD=1.33%）. Conclusion： The method is simple, accurate, reproducible and can control the quality of Fukang lotion effectively. |
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| Keywords: | Fukang lotion TLC HPLC oxymatrine matrine quality standard |
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