| 斑点金免疫渗滤法测定人全血中抗HBc抗体 |
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| 引用本文： | 邓为民,徐世文,潘菊芬. 斑点金免疫渗滤法测定人全血中抗HBc抗体[J]. 中国免疫学杂志, 1999, 15(12): 572-573 |
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| 作者姓名： | 邓为民 徐世文 潘菊芬 |
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| 作者单位： | 天津医科大学免疫学教研室,天津,300070 |
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| 摘 要： | 目的：建立一种适合斑点免疫渗滤法（Ｄｏｔ Ｉｍｍｕ ｎｏ ｇｏｌｄ Ｆｉｌｔｒａｔｉｏｎ Ａｓｓａｙ ＤＩＧＦＡ）特点的快速全血诊断方法，方法：选择可瞬间分离血中细胞和血清的膜，依据ＤＩＧＦＡ的原理对３３份ＨＢｃＡｂ阳性血样和４８份阴性血样进行了全血抗ＨＢｃＡｇ抗体的检测。结果：测定阳性标本率为９６．７％，阴性标本符合率为１００％。结论：提供了一种简便、快速、可行的全血检测抗ＨＢｃＡｇ抗体的方法。
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| 关 键 词： | 抗HBc Ab 全血 DIGFA |
Detecting anti-HBc antibody in whole blood by dot immunogold filitration assay |
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| DENG Wei-Min,XU Shi-Wen,PAN Ju-Fen. Detecting anti-HBc antibody in whole blood by dot immunogold filitration assay[J]. Chinese Journal of Immunology, 1999, 15(12): 572-573 |
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| Authors: | DENG Wei-Min XU Shi-Wen PAN Ju-Fen |
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| Abstract: | Objective:TO establish a rapid, simple and reliable assay for detecting anti-HBc Ab in human whole blood.Methods: A newdot immunogold filtration assay was selected. Result: In detecting 33 anti-HBc Ah positive samples and 48 negative samples confirmed by clinical indexes, the positive coincidence rate was 96.7%; and negative late was 100% Conclusion:This method not only has the characteristicsof rapidness,simpleness and reliability, but the has an obvious advantage, that we could detect the anti-HBc Ab in whole bind, but not inserum, and obtain the result immediately. |
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| Keywords: | DIGFA Anti-HBc Ab Whole blood |
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