2010-2013年湖南省市售水产品中副溶血性弧菌污染状况及病原特征分析

目的 了解湖南省市售水产品中副溶血性弧菌污染状况和病原特征。 方法 2010-2013年在湖南省14个市的集贸市场和超市,按照无菌采样原则采集动物性水产品1 263份,分离副溶血性弧菌,进行毒力基因、耐药表型和脉冲场凝胶电泳(PFGE)分子分型。 结果 1 263份水产品中,221份样品检出副溶血性弧菌,检出率为17.50%。3株副溶血性弧菌检出trh基因,1株检出tdh基因,毒力基因携带率为1.81%。分离菌株对氨苄青霉素耐药率最高,达30.32%。其次为对氯霉素(1.81%)、复方新诺明(1.36%)和四环素(0.45%),未发现多重耐药菌株。分离菌株经NotⅠ酶切后,脉冲场凝胶电泳带型相对分散。 结论 2010-2013年湖南省市售水产品中副溶血性弧菌污染率高,部分菌株携带毒力基因,并具有一定的耐药性。

Contamination status and pathogenic characteristics of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province, 2010-2013

Objective To investigate the contamination status and pathogenic features of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province. Methods According to the aseptic sampling principle, 1,263 aquatic products of animal origin were collected from fair markets and supermarkets in 14 cities of Hunan Province. Vibrio parahaemolyticus was isolated and subjected to virulence gene detection, antimicrobial susceptibility testing and molecular typing by pulsed field gel electrophoresis (PFGE). Results Vibrio parahaemolyticus was detected in 221 of 1,263 aquatic products, with the detection rate of 17.50%. The trh gene was positive in 3 isolates and tdh gene in 1 isolate, with the virulence gene carrying rate of 1.81%. The isolates were the most resistant to ampicillin (30.32%), then to chloramphenicol (1.81%), sulfamethoxazole compound (1.36%) and tetracycline (0.45%). No multidrug resistance strain was detected. PFGE indicated that the bands of the isolates digested by Not I were relatively dispersed. Conclusions The contamination of Vibrio parahaemolyticus in aquatic products sold in cities of Hunan Province is severe, and some isolates carry virulence gene and express resistance to antibiotics.