Contribution of momilactones A and B to diabetes inhibitory potential of rice bran: Evidence from in vitro assays |
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| Authors: | Nguyen Van Quan Tran Dang Xuan Hoang-Dung Tran Ateeque Ahmad Tran Dang Khanh Tran Dang Dat |
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| Affiliation: | 1. Division of Development Technology, Graduate School for International Development and Cooperation (IDEC), Hiroshima University, Higashi Hiroshima 739-8529, Japan;2. Department of Biotechnology, NTT Institute of Hi-Technology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street,Ward 13, District 4, Ho Chi Minh 72820, Viet Nam;3. Process Chemistry and Technology Department, Central Institute of Medicinal and Aromatic Plants, Lucknow 226016, India;4. Agricultural Genetics Institute, Pham Van Dong Street, Hanoi 122000, Viet Nam;5. Center for Expert, Vietnam National University of Agriculture, Hanoi 131000, Viet Nam;6. Khai Xuan International Co. Ltd., Ha Dong District, Duong Noi Ward, LK20A-20B, Khai Xuan Building, Hanoi 152611, Viet Nam |
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| Abstract: | This study was the first to detect the presence of the two compounds momilactone A (MA) and momilactone B (MB) in rice bran using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). By in vitro assays, both MA and MB exhibited potent inhibitory activities on pancreatic α-amylase and α-glucosidase which were significantly higher than γ-oryzanol, a well-known diabetes inhibitor. Remarkably, MA and MB indicated an effective inhibition on trypsin with the IC50 values of 921.55 and 884.03 µg/mL, respectively. By high-performance liquid chromatography (HPLC), quantities of MA (6.65 µg/g dry weight) and MB (6.24 µg/g dry weight) in rice bran were determined. Findings of this study revealed the α-amylase, α-glucosidase and trypsin inhibitors MA and MB contributed an active role to the diabetes inhibitory potential of rice bran. |
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| Keywords: | Corresponding author. Momilactones A and B Rice bran Anti-diabetes α-amylase α-glucosidase Trypsin HPLC and LC-ESI-MS methods |
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