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肉苁蓉花序挥发物的GC-MS成分分析
引用本文:乔海莉,陆鹏飞,徐荣,陈君,王霞,马维思,刘同宁.肉苁蓉花序挥发物的GC-MS成分分析[J].中药材,2012(4):573-577.
作者姓名:乔海莉  陆鹏飞  徐荣  陈君  王霞  马维思  刘同宁
作者单位:中国医学科学院北京协和医学院药用植物研究所;北京林业大学省部共建森林培育与保护教育部重点实验室;宁夏永宁县本草苁蓉种植基地
基金项目:国家自然科学基金项目(81102747,81102748);国家科技支撑计划项目(2011BAI05B03);宁夏回族自治区科技攻关计划项目(YKX-11-116)
摘    要:目的:研究肉苁蓉花序挥发物的种类和相对含量,为其综合利用和种子繁育提供依据。方法:利用动态顶空套袋吸附法和气相色谱-质谱联用分析方法,研究近自然状态下肉苁蓉花期花序挥发物成分。结果:现蕾期到盛花期共从肉苁蓉花序中鉴定出40种挥发性化合物。现蕾期主要为烃类与绿叶挥发物,随着肉苁蓉花序上花朵开放,部分挥发物相对含量消失或减少,部分化合物相对含量增加,并出现一些新化合物。初花期和盛花期肉苁蓉花序主要挥发性成分为芳香性酯类与苯环类化合物,其相对含量较现蕾期显著增加。结论:肉苁蓉花序的挥发性成分种类多,且随开花进程出现种类和相对含量的变化,构成了肉苁蓉不同花期的香韵。

关 键 词:肉苁蓉花序  挥发物  动态顶空套袋吸附法  气相色谱-质谱联用

Analysis of Volatile Compounds of Inflorescence by GC-MS from Cistanche deserticola
QIAO Hai-li,LU Peng-fei,XU Rong,CHEN Jun,WANG Xia,MA Wei-si,LIU Tong-ning.Analysis of Volatile Compounds of Inflorescence by GC-MS from Cistanche deserticola[J].Jorunal of Chinese Medicinal Materials,2012(4):573-577.
Authors:QIAO Hai-li  LU Peng-fei  XU Rong  CHEN Jun  WANG Xia  MA Wei-si  LIU Tong-ning
Institution:1.Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100193,China;2.Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,China;3.Ningxia Yongning Bencao Plantation of Cistanche,Yinchuan 750100,China)
Abstract:Objective:To study the volatile compounds from inflorescence of Cistanche deserticola and provide basis for its utilization and seed breeding.Methods:The volatile compounds were collected by dynamic headspace adsorption and analyzed by gas chromatography-mass spectrometry(GC-MS).Results:Forty volatile components were identified in inflorescence of Cistanche deserticola from squaring period to full-bloom period.The main components in buds of Cistanche deserticola were hydrocarbons and green leaf volatiles in squaring period.Some components were characteristic in buds and disappeared or decreased in flowers.The relative contents of some components gradually increased with the buds blooming.And some components only emerged in flowers of Cistanche deserticola.The higher content of esters and aromatics were found in flowers,which were significantly increased in comparison with the volatile compounds from buds.Conclusion:The volatile compounds from inflorescence of Cistanche deserticola were complex,consisting of various compositions and significantly different with buds blooming.
Keywords:Inflorescence of Cistanche deserticola Y  C  Ma  Volatile compounds  Dynamic headspace adsorption  Gas chromatography-mass spectrometry
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