| C57BL/6小鼠内耳形态学和年龄相关性听力损失的研究 |
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| 引用本文： | 徐延军,杨仕明,胡吟燕,徐金操,孙建和,李兴启,翟所强,韩东一. C57BL/6小鼠内耳形态学和年龄相关性听力损失的研究[J]. 中华耳科学杂志, 2008, 6(4) |
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| 作者姓名： | 徐延军 杨仕明 胡吟燕 徐金操 孙建和 李兴启 翟所强 韩东一 |
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| 作者单位： | 解放军总医院耳鼻咽喉-头颈外科,解放军耳鼻咽喉研究所耳神经生物中心,北京,100853 |
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| 基金项目： | 国家自然科学基金，海外青年学者合作基金，北京市自然科学基金，国家高技术研究发展计划(863计划)，国家自然科学基金重点项目 |
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| 摘 要： | 目的探讨不同周龄C57BL/6小鼠内耳形态学及其ABR阈值变化。方法取C57BL/6小鼠3周、4周、12周、26周各10只,听性脑干反应(ABR)测试双侧2、4、8、16、20 kHz ABR阈值。采用基底膜铺片MyosinⅥ、Neurofilament免疫组化染色,观察耳蜗毛细胞和神经丝的变化。扫描电镜观察耳蜗毛细胞及其静纤毛随年龄的变化。结果随着年龄增长,C57BL/6小鼠各频率ABR阈值明显提高,顶转和底转内毛细胞缺失逐渐增多,神经丝染色渐淡,毛细胞静纤毛逐渐发生数量减少、增粗融合、倒伏等变化。到26周龄时已达到重度聋,各频率较3周组有显著统计学差异。顶转和底转内毛细胞有连续缺失,外毛细胞完全缺失,内毛细胞只有残存的少量静纤毛,粗细不均,倒伏明显。结论本研究对国产C57BL/6小鼠的内耳形态进行观察,明确了其ABR阈值和内耳毛细胞的变化规律,为用国产C57BL/6小鼠进行老年性聋研究提供了依据。
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| 关 键 词： | C57BL/6小鼠 耳蜗 年龄相关性听力损失 毛细胞 |
Age-related hearing loss and inner ear morphological changes in C57BL/6 mice |
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| XU Yan-jun,YANG Shi-ming,HU Yin-yan,XU Jin-cao,SUN Jian-he,LI Xing-qi,ZHAI Suo-qiang,HAN Dong-yi. Age-related hearing loss and inner ear morphological changes in C57BL/6 mice[J]. Chinese Journal of Otology, 2008, 6(4) |
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| Authors: | XU Yan-jun YANG Shi-ming HU Yin-yan XU Jin-cao SUN Jian-he LI Xing-qi ZHAI Suo-qiang HAN Dong-yi |
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| Affiliation: | XU Yan-jun,YANG Shi-ming,HU Yin-yan,XU Jin-cao,SUN Jian-he,LI Xing-qi,ZHAI Suo-qiang,HAN Dong-yi Department of Otolaryngology , Head & Neck Surgery,Otolaryngology Institute of Chinese PLA General Hospital,Beijing 100853,China |
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| Abstract: | Objective To study hearing and inner ear morphology changes in inbred C57BL/6 mice at various ages.Methods Binaural ABR thresholds at 2,4,8,16 and 20 kHz were evaluated in C57BL/6 mice of 3,4,12 and 26 weeks of age.Morphological changes of the inner ear were studied using scanning electron microscopy and staining of Myosin VI and Neurofilament.Results Binaural ABR thresholds gradually increased along with age.ABR thresholds at 26 weeks were significantly higher at all frequencies than at 3 weeks(P < 0.01).M... |
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| Keywords: | C57BL/6 mice Cochlea Age-related hearing loss Hair cell |
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