连钱草乙醇提取物对豚鼠离体肠平滑肌和小鼠肠运动功能的影响

目的:观察连钱草乙醇提取物对小鼠小肠推进运动、药物性腹泻小鼠模型和豚鼠离体回肠平滑肌收缩的影响,探讨其作用机理.方法:采用在体方法观察连钱草乙醇提取物对小鼠小肠推进功能的影响,采用离体方法观察连钱草乙醇提取物对豚鼠回肠平滑肌运动功能的影响.结果:连钱草乙醇提取物能够显著抑制小鼠小肠炭末推进率(P＜0.01),缓解大黄所致小鼠腹泻 (P＜0.01),对抗新斯的明所致的肠蠕动亢进 (P＜0.01).抑制豚鼠离体回肠平滑肌收缩(P＜0.01),拮抗乙酰胆碱、组胺、氯化钡对离体豚鼠回肠平滑肌的激动作用(P＜0.01).结论:连钱草乙醇提取物具有抑制肠蠕动作用,这种作用可能由胃肠道的胆碱能受体和组胺受体介导,或直接作用于回肠平滑肌细胞.

Effect of the ethanol extracts of Glechoma longituba on isolated ileum smooth muscle of guinea pig and the intestine movement function of mice

OBJECTIVE To study the effect and mechanisms of the ethanol extracts of Glechoma longituba (Nakai) Kupr (EGLK) on the small intestine propulsive function in mice, diarrhea models induced by the water extracts of rhubarb and neostigmine methylsulfate and the isolated ileum smooth muscle movement. METHODS The experiment method of intestine propulsion of carbon ink in mice, diarrhea model induced by the water extracts of rhubarb and neostigmine methylsulfate and the experiment method of isolated ileum smooth muscle of guinea pig were used in the experiments. RESULTS The intestinal propelling movement of carbon ink and experimental diarrhea caused by the water extracts of rhubarb and neostigmine methylsulfate in mice could be markedly antagonized by EGLK. The spontaneous movements of isolated intestinal smooth muscle in guinea pig could be suppressed. The excitation induced by acetylcholine, histamine and BaCL 2 could be also antagonized.CONCLUSIONS EGLK has the inhibition effect on intestine peristalsis which may be mediated through cholinoceptor and histamine receptor or directly acts on the cells of ileum smooth muscle.