利用基因芯片技术快速检测结核杆菌耐药性的初步研究

目的：探讨利用基因芯片技术检测结核杆菌的耐药性。方法：结核杆菌耐药性检测基因芯片的制备；临床分离株结核杆菌培养和药敏检测；结核杆菌基因组DNA的制备和结核杆菌耐药基因的聚和酶链反应；基因芯片的杂交、洗涤、检测与分析。结果：临床分离株结核杆菌培养和药敏检测结果：1株为药物敏感株结核杆菌(对异烟肼、利福平、链霉素、乙肤丁醇4种药物均敏感)；4株为多耐药株结核杆菌(对异烟肼、利福平、链霉素、乙肤丁醇4种药物均耐药)；药敏检测结果与5位患者临床的诊断性治疗的结果完全一致；进一步利用基因芯片技术进行检测，检测结果与上述结果相符合。结论：利用基因芯片技术检测结核杆菌的耐药性，准确、快速、高效。

Preliminary study on detecting drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization

AIM:To detect the drug-resistance mycobacterium tuberculosis by using DNA microarray hybridization.METHODS: DNA microarray for detecting drug-resistance of mycobacterium tuberculosis was prepared; Clinical isolated strains were cultivated and their drug-resistance sensitivity was detected. The genome DNA of mycobacterium tuberculosis was prepared and the drug-resistance genes of the mycobacterium tuberculosis were amplified by PCR. Then the gene chip was hybridized, washed, detected and analyzed. RESULTS: Results of cultivating mycobacterium tuberculosis and detecting the drug-resistance sensitivity: one strain was drug-sensitive; four strains were multi-drug-resistant; The detecting results of the drug-resistance was consistent with the results of diagnosis therapy of the 5 clinical patients. The detecting results of gene chip confirmed the above facts. CONCLUSION: Detecting drug-resistance mycobacterium tuberculosis by the gene chip is precise, fast and highly-efficient.