噪声损伤后豚鼠耳蜗核复合体一氧化氮合酶阳性神经元的变化

目的 :探讨噪声对豚鼠耳蜗核复合体 (CNC)内一氧化氮合酶 (NOS)阳性神经元数目和面积的影响。方法 :采用依赖还原型辅酶II的尼克酰胺腺嘌呤二核苷酸磷酸黄递酶 (NADPH d)组织化学方法 ,观察正常组和噪声损伤组 (噪声损伤后 1d ,1周 ,4周 )CNC内NOS阳性神经元数目和面积的变化。结果 :与正常组相比 ,噪声损伤后 1d至 4周 ,CNC内NOS阳性神经元数目逐渐增多 ;噪声损伤后 1dNOS阳性神经元面积急剧缩小 ,噪声损伤后 1～ 4周NOS阳性神经元面积逐渐升高并超过正常水平。与正常组比较 ,噪声损伤后 1d ,1周 ,4周组 ,NOS阳性神经元面积和数目差异有显著性 (P <0 .0 5 ) ,噪声损伤后 1d ,1周 ,4周 3组之间差异亦有显著性 (P <0 .0 5 )。结论 :在噪声损伤后 ,NO释放增多 ,对听觉核团有复杂的调节作用

The change of nitric oxide positive neurons in cochlear nucleus complex of guinea pigs with noise induced deafness

Aim: To study the expression of nitric oxide synthetase (NOS) positive neurons(number and area) in cochlear nucleus complex (CNC) of guinea pigs with temporary noise induced deafness. Methods: Nicotinamidc adenine dinucleotide phosphate diaphorase (NADPH d) histochemistry method was adopted to study the expression of NOS positive neurons(number and area) in CNC in normal group and 3 noise induced deafness groups (alive 1 d, 1 week and 4 weeks after exposed to the strong noise). Results: Compared with normal group, the number of NOS positive neurons in CNC increased during the period from 1d to 4weeks after the noise exposure. The area of NOS positive neurons in CNC first decreased promptly (1d after the noise exposure) and almost continuously increased afterwards. There were significant differences not only between the noise induced deafness groups and normal group( P <0.05) but between the 3 noise induced deafness groups( P <0.05). Conclusion: It suggests that the release of NO increases after noise exposure.NO has complex regulative effects on CNC.