| 血管生成素2促进缺血心肌细胞凋亡 |
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| 引用本文： | 蒙荣森,陈波,董吁钢. 血管生成素2促进缺血心肌细胞凋亡[J]. 中国医药指南, 2008, 6(24): 426-429 |
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| 作者姓名： | 蒙荣森 陈波 董吁钢 |
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| 作者单位： | 1. 广州中山大学附属第一医院心血管医学部,510080
2. 美国Georgia医学院 |
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| 摘 要： | 目的探讨血管紧张素对缺血部位心肌细胞凋亡的影响及可能机制。方法实验动物分假手术组、模型组和实验组。细胞凋亡情况采用原位缺口末端标记法及流式细胞仪Annexin V/PI双标检测法,检测心肌细胞凋亡情况,并通过TR-PCR法检测Bcl-xl和Caspase-3的mRNA表达。结果与假手术组比较模型组凋亡细胞增多,实验组凋亡的细胞数进一步增加。模型组Bcl-xl的mRNA表达下降,实验组下降更明显及模型组Caspase-3的mRNA表达增高,实验组表达最高。结论血管生成素2促进缺血心肌细胞凋亡,其机制可能与抑制Bcl-xl表达及促进Caspase-3表达有关。
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| 关 键 词： | 血管生成素2 心肌细胞 凋亡 |
Angiopoietin-2 induced ischemic cardiomyocytes apoptosis |
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| Rong-Sen Meng,Bo Chen,Yu-Gang Dong. Angiopoietin-2 induced ischemic cardiomyocytes apoptosis[J]. Guide of China Medicine, 2008, 6(24): 426-429 |
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| Authors: | Rong-Sen Meng Bo Chen Yu-Gang Dong |
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| Affiliation: | Rong-Sen Meng~1,Bo Chen~2,Yu-Gang Dong~1 The First Affiliated Hospital,SunYat-Sen University,guangzhou,510080,China. The medical college of Georgia USA. |
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| Abstract: | Objective To investigate the effects of angiopoietin-2 on ischemic cardiomyocytes apoptosis and its mechanism.Methods Experimental animals were divided into three groups:sham,model and experimental group.Cellular apoptosis were measured by terminal deoxynucleotidyl transferease-mediated nick end labelling(TUNEL)and flow cytomehry(Annexin V/PI).The expressions of mRNA of Bcl-xl and Caspase-3 were measured by RT-PCR.Results Cellular apoptosis in model group was higher than sham group,and Cellular apoptosis in... |
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| Keywords: | Angiopoietin-2 Cardiomyocytes Apoptosis |
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