| 温热处理对K562细胞系bcr／abl融合基因mRNA转录和Caspase-3表达的影响 |
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| 引用本文： | 高炳华,王新生,焦保良,张斌,范凌,刘洁,薄爱华. 温热处理对K562细胞系bcr／abl融合基因mRNA转录和Caspase-3表达的影响[J]. 中国微循环, 2009, 13(6): 485-487 |
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| 作者姓名： | 高炳华 王新生 焦保良 张斌 范凌 刘洁 薄爱华 |
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| 作者单位： | 1. 河北北方学院附属第一医院血液科,河北张家口,075000
2. 河北北方学院第一临床医学院 |
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| 摘 要： | 目的观察不同温度刺激对K562细胞bcr／abl融合基因mRNA转录和Caspase-3蛋白表达的影响。方法将培养获得的K562细胞分别进行40℃、43℃、46℃恒温刺激30min，以37％作为对照；RT-PCR技术检测bcr／abl融合基因mRNA的转录，Westernbloting技术检测Caspase-3的表达。结果K562细胞热刺激30min后，bcr／abl融合基因mRNA的转录随温度升高而下调，Caspase-3的表达随温度升高而上调。结论温热刺激可降低K562细胞内bcr／abl融合基因mRNA的转录，提高Caspase-3的表达。
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| 关 键 词： | 热疗 K562细胞系 Caspase-3 bcr/abl融合基因 |
Effect of Heat Stimulation on the Expression of Caspase-3 and the Transcriptional Levels of bcr/abl Fusion Gene mRNA in K562 Cell Line |
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| GAO Bing-hua,WANG Xin-sheng,JIAO Bao-liang,ZHANG Bin,FAN Ling,LIU Jie,BO Ai-hua. Effect of Heat Stimulation on the Expression of Caspase-3 and the Transcriptional Levels of bcr/abl Fusion Gene mRNA in K562 Cell Line[J]. Journal of Chinese Microcirculation, 2009, 13(6): 485-487 |
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| Authors: | GAO Bing-hua WANG Xin-sheng JIAO Bao-liang ZHANG Bin FAN Ling LIU Jie BO Ai-hua |
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| Affiliation: | GAO Bing-hua, ZHANG Bin, FAN Ling, LIU Jie,WANG Xin-sheng, JIAO Bao-liang, BO Ai-hua.( Department of Haematology, The First Affiliated Hospital, Hebei North University, Zhangjiakou 075000, China; 2. The First Clinical Medical School, Hebei North University, Zhangjiakou 075000, China ) |
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| Abstract: | |
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| Keywords: | Caspase-3 |
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