Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G2-phase arrest |
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Authors: | Chen Hong-ying Zheng Cong-yi Zou Guo-lin Xie Da-xing Gong Jian-ping |
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Institution: | [1]CollegeofLifeSciences,WuhanUniversity,Wuhan430072 [2]CancerResearchInstitute,TongjiHospital,TongiiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430072,China |
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Abstract: | AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402). METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 mumol/L for 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest. |
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Keywords: | peplomycin Bel-7402 cells apoptosis cyclins cell cycle flow cytometry |
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