中华眼镜蛇毒活性组分选择性抑制内皮细胞增殖

目的观察中华眼镜蛇毒活性组分(Ch ina cobra venomactive factor,CCVAF)对内皮细胞增殖的选择性抑制作用。方法实验分对照组和不同浓度的CCVAF组,应用MTT法分析CCVAF对牛肺主动脉内皮细胞(bovine arteria pu lmona-lis vascu lar endothelial cells,BAVEC)、SD大鼠主动脉血管平滑肌细胞(smooth musc le cells,SMC)、人胚肺成纤维细胞HLF以及人支气管上皮细胞HBE生长增殖的影响;并运用直线回归分析统计对比CCVAF在不同时间点对BAVEC的IC50及上述各细胞的24 h IC50。结果CCVAF呈剂量及时间依赖性抑制内皮细胞的增殖:6、12、24、48、72 h其IC50分别为4.955、4.932、2.443、3.048、3.133 mg.L-1,24 h抑制作用最强;观察此时浓度与抑制作用的关系,0.625、1.25、2.5、5、10、20 mg.L-1,抑制率分别是4.41%、30.94%、61.81%、86.17%、96.05%、98.68%,10 mg.L-1抑制率即达高峰,且各浓度组抑制作用与对照组相比差异有显著性(P<0.05)。CCVAF抑制内皮细胞增殖具有一定的选择性,作用24 h后,抑制BAVEC生长的作用明显高于其它3种细胞,对BAVEC、HLF、HBE、SMC的24 h IC50分别为2.443、22.233、35.318、32.810 mg.L-1。结论CCVAF能够选择性抑制内皮细胞增殖,这在抗血管生成及其靶向治疗研究中可能具有重要意义。

Selective inhibition effect of China cobra venom active factor on endothelial cells

Aim To investigate selective inhibition effect of China cobra venom active factor(CCVAF) from China cobra venom on endothelia cells.Methods There were control group and CCVAF groups(different concentrations) in the experiment.With different CCVAF concentrations,the proliferation of bovine arteria pulmonalis vascular endothelial cells(BAVEC),SD rats aorta smooth muscle cells(SMC),human embryonic lung fibroblast(HLF) and human bronchial epithelium cells(HBE) were observed by MTT methods,and cell proliferation inhibition IC_(50) of CCVAF against the above different cell strains were calculated by linear regression analysis system.Results CCVAF exhibited significantly time-dependent inhibitive effect on the proliferation of BAVEC,IC_(50) of CCVAF on BAVEC at 6,12,24,48 and 72 h after coincubation were 4.955,4.932,2.443,3.048 and 3.133 mg·L~(-1),respectively,and reached the peak at 24 h;at the same time,CCVAF inhibited the proliferation of BAVEC in dose-dependent maner,inhibition rates of different doses of CCVAF(ie 0.625,1.25,2.5,5,10 and 20 mg·L~(-1)) on BAVEC were 4.41%,30.94%,61.81%,86.17%,96.05% and 98.68%,respectively.There were significant differences between all CCVAF groups and control group(P<0.05).The inhibition rates reached peak at 10 mg·L~(-1).Our data also indicated that CCVAF selectively inhibited the proliferation of endothelial cells,IC_(50) of CCVAF on BAVEC,SMC,HLF and HBE were 2.443,22.233,35.318 and 32.810 mg·L~(1) at 24 h after incubation.Conclusion CCVAF can selectively inhibit the proliferation of endothelial cells,which lays a foundation for further developmetn of anti-tumor drugs target at endothelial cells.