肾血管性高血压对诱导型一氧化氮合酶表达及活性的影响

目的通过测定肾血管性高血压大鼠血管及肾组织诱导型一氧化氮合酶（iNOS）活性及表达的变化情况，探讨血压与iNOS间的关系。方法运用肾动脉不全结扎方法制备SD大鼠肾血管性高血压模型，并应用Greiss反应、L-精氨酸同位素标记法及Westernblot等分别测定一氧化氮的终产物——尿中NO

Effect of renovascular hypertension on activity and expression of inducible nitric oxide synthase

Objective:To investigate the relationship between arterial pressure and expression as well as activity of inducible nitric oxide synthase (iNOS).Methods:The renovascular hypertension was reproduced by renal artery stenosis in Spraugue Dawley (SD) rats.Levels of UNOx,activity and protein expression of iNOS were measured by Greiss reaction,the conversion rate of isotope-labelled L-arginine to citrulline and Western blot respectively.Results:Following renal artery stenosis,UNOx,an index of the nitric oxide(NO) production of the whole body,significantly increased at one week after operation (P<0.01),it increased to (5 451.4±795.2)nmol/24 h from(2 307.3±486.6)nmol/24 h prior to operation.It was also observed that the iNOS activity significantly increased in aorta and renal tissues including cortex and medulla of renovascular hypertensive rats compared with controls.Western blot showed iNOS expression was higher in aorta (236.7%),but not in renal medulla of renovascular hypertensive rats than that of controls.Conclusions:iNOS/NO pathway plays an important role in regulation of arterial pressure in renovascular hypertension.iNOS could be activated in case of hypertension by a pressure-related mechanism.